ATG3 blocking peptide

ATG3 Antibody (N-term) Blocking peptide

• Gene Names: ATG3; APG3; APG3L; PC3-96; APG3-LIKE
• Synonyms: ATG3; ATG3 Antibody (N-term) Blocking peptide; Ubiquitin-like-conjugating enzyme ATG3; 632-; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; APG3; APG3L; ATG3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human Autophagy APG3L. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 314

• Cellular Location: Cytoplasm.
• Tissue Location: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATG3 blocking peptide

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 61212142
• NCBI GeneID: 64422
• UniProt Accession #: Q9NT62; Q6PKC5; Q9H6L9
• Molecular Weight: 35,467 Da
• NCBI Official Full Name: Ubiquitin-like-conjugating enzyme ATG3
• NCBI Official Synonym Full Names: autophagy related 3
• NCBI Official Symbol: ATG3
• NCBI Official Synonym Symbols: APG3; APG3L; PC3-96; APG3-LIKE
• NCBI Protein Information: ubiquitin-like-conjugating enzyme ATG3
• UniProt Protein Name: Ubiquitin-like-conjugating enzyme ATG3
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG3
• UniProt Synonym Gene Names: APG3; APG3L; APG3-like; hApg3
• UniProt Entry Name: ATG3_HUMAN

NCBI Description

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG3: an E2-like enzyme essential for Apg8p lipidation.

Protein type: EC 6.3.2.-; Autophagy; Ubiquitin conjugating system; Enzyme, cellular metabolism

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol

Molecular Function: APG12 conjugating enzyme activity; APG8 conjugating enzyme activity; enzyme binding; protein binding; small conjugating protein ligase activity

Biological Process: autophagic vacuole formation; macroautophagy; mitochondrial fragmentation during apoptosis; mitochondrion degradation; protein modification process; protein targeting to membrane; protein ubiquitination