ASPM blocking peptide

ASPM Blocking Peptide

Cat. Num. AAA9620400

• Gene Names: ASPM; ASP; MCPH5; Calmbp1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ASPM; ASPM Blocking Peptide; ASPM blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ASPM blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ASPM blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 332205965
• NCBI GeneID: 259266
• NCBI Accession #: NP_001193775.1
• NCBI GenBank Nucleotide #: NM_001206846.1
• UniProt Accession #: Q8IZT6; Q4G1H1; Q5VYL3; Q86UX4; Q8IUL2; Q8IZJ7; Q8IZJ8; Q8IZJ9; Q8N4D1; Q9NVS1; Q9NVT6
• Molecular Weight: 409,800 Da
• NCBI Official Full Name: abnormal spindle-like microcephaly-associated protein isoform 2
• NCBI Official Synonym Full Names: asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
• NCBI Official Symbol: ASPM
• NCBI Official Synonym Symbols: ASP; MCPH5; Calmbp1
• NCBI Protein Information: abnormal spindle-like microcephaly-associated protein
• UniProt Protein Name: Abnormal spindle-like microcephaly-associated protein
• Protein Family: Abnormal spindle-like microcephaly-associated protein
• UniProt Gene Name: ASPM
• UniProt Synonym Gene Names: MCPH5; Asp homolog
• UniProt Entry Name: ASPM_HUMAN

NCBI Description

This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

ASPM: Probable role in mitotic spindle regulation and coordination of mitotic processes. May have a preferential role in regulating neurogenesis. Defects in ASPM are the cause of microcephaly primary type 5 (MCPH5); also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: microtubule; cytoplasm; midbody; nucleus

Molecular Function: calmodulin binding

Biological Process: oogenesis; positive regulation of neuroblast proliferation; mitosis; developmental growth; maintenance of centrosome localization; negative regulation of neuron differentiation; negative regulation of asymmetric cell division; male gonad development; neuron migration; spermatogenesis; cerebral cortex development; forebrain neuroblast division

Disease: Microcephaly 5, Primary, Autosomal Recessive