ALK blocking peptide

ALK Antibody (Center) Blocking Peptide

• Gene Names: ALK; CD246; NBLST3
• Synonyms: ALK; ALK Antibody (Center) Blocking Peptide; ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; CD246; ALK blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human ALK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1620

• Cellular Location: Cell membrane; Single-pass type I membrane protein Note: Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway
• Tissue Location: Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ALK blocking peptide

Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

NCBI and Uniprot Product Information

• NCBI GI #: 296439447
• NCBI GeneID: 238
• UniProt Accession #: Q9UM73; Q4ZFX9; Q53QQ6; Q53RZ4; Q59FI3; Q9Y4K6
• Molecular Weight: 176,442 Da
• NCBI Official Full Name: ALK tyrosine kinase receptor
• NCBI Official Synonym Full Names: anaplastic lymphoma receptor tyrosine kinase
• NCBI Official Symbol: ALK
• NCBI Official Synonym Symbols: CD246; NBLST3
• NCBI Protein Information: ALK tyrosine kinase receptor
• UniProt Protein Name: ALK tyrosine kinase receptor
• Protein Family: Alkylsulfatase
• UniProt Gene Name: ALK
• UniProt Entry Name: ALK_HUMAN

NCBI Description

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Uniprot Description

ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors

Protein type: Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Membrane protein, integral; Kinase, protein; TK group; Alk family

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: integral to plasma membrane; protein complex

Molecular Function: identical protein binding; NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; cell proliferation; neuron development; phosphorylation; protein amino acid autophosphorylation; regulation of apoptosis; signal transduction

Disease: Neuroblastoma, Susceptibility To, 3