Antibody Coating Buffer Blocking Buffer
Antibody Coating Buffer, 5X
Synonyms
Antibody Coating Buffer; 5X; Antibody Coating Buffer blocking buffer
Form/Format
Supplied at 5X.
Application Notes
1. Dilute 5X Antibody Coating Buffer 1:5 with deionized water and mix for 15 minutes. As Antibody Coating Buffer is a 5X concentrate, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm the buffer until all crystals are dissolved. Do not let it boil.
2. Dilute your antibody into the coating buffer. Optimal coating concentration varies significantly from 0.1 ug/mL to 10 ug/mL.
3. Let the solution stir 10-15 minutes and pipette onto the plate. Optimal coating volume generally ranges between 50-200 uL per well. ICT recommends coating antibodies onto Costar 96-Well EIA/RIA Stripwell plate.
4. Once added to the plate, incubate the coating solution from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering.
5. Aspirate the coating solution.
6. Wash each well twice with 1X ELISA Wash Buffer.
7. Block the uncoated regions of the microplate wells by pipetting 300 muL of blocking buffer into each well.
8. Incubate 8-24 hours at room temperature.
9. Aspirate the blocking buffer.
10. The assay can be run at this point, or the plate can be dried and packaged for later use.
11. Dry the plate by letting it sit on the bench top from 8-24 hours while protected from light, or dry in a drying chamber under vacuum from 3-6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant packet and store at RT or 2-8 degree C protected from light.
2. Dilute your antibody into the coating buffer. Optimal coating concentration varies significantly from 0.1 ug/mL to 10 ug/mL.
3. Let the solution stir 10-15 minutes and pipette onto the plate. Optimal coating volume generally ranges between 50-200 uL per well. ICT recommends coating antibodies onto Costar 96-Well EIA/RIA Stripwell plate.
4. Once added to the plate, incubate the coating solution from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering.
5. Aspirate the coating solution.
6. Wash each well twice with 1X ELISA Wash Buffer.
7. Block the uncoated regions of the microplate wells by pipetting 300 muL of blocking buffer into each well.
8. Incubate 8-24 hours at room temperature.
9. Aspirate the blocking buffer.
10. The assay can be run at this point, or the plate can be dried and packaged for later use.
11. Dry the plate by letting it sit on the bench top from 8-24 hours while protected from light, or dry in a drying chamber under vacuum from 3-6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant packet and store at RT or 2-8 degree C protected from light.
Contains
No proteins
Preparation and Storage
Store at room temperature or 2-8 degree C.
Related Product Information for Antibody Coating Buffer blocking buffer
Description: Use Antibody Coating Buffer to save reagents, enhance specific signal, and extend the shelf life of coated plates. Antibody Coating Buffer maximizes the adsorption of antibodies onto polystyrene plates and stabilizes the three-dimensional structure of the antibody for optimal performance.
Background: Antibody Coating Buffer, 5X maximizes the adsorption of monoclonal and polyclonal antibodies onto polystyrene plates for use in capture (sandwich) immunoassays by stabilizing the three-dimensional structure of the antibody for optimal immunoassay performance.
Stabilizing the adsorbed antibody with Antibody Coating Buffer also preserves the antigen recognition regions of the antibody. This allows for greater binding reactivity with the target antigen and the detection molecule, thus enhancing the specific signal. By generating a higher specific signal, a lower concentration of coating antibody may be used, saving valuable reagents.Under proper storage conditions, coated ELISA /EIA /RIA plates may last for years, enabling the preparation of large batches to be stored for future experiments.
Background: Antibody Coating Buffer, 5X maximizes the adsorption of monoclonal and polyclonal antibodies onto polystyrene plates for use in capture (sandwich) immunoassays by stabilizing the three-dimensional structure of the antibody for optimal immunoassay performance.
Stabilizing the adsorbed antibody with Antibody Coating Buffer also preserves the antigen recognition regions of the antibody. This allows for greater binding reactivity with the target antigen and the detection molecule, thus enhancing the specific signal. By generating a higher specific signal, a lower concentration of coating antibody may be used, saving valuable reagents.Under proper storage conditions, coated ELISA /EIA /RIA plates may last for years, enabling the preparation of large batches to be stored for future experiments.
Product Categories/Family for Antibody Coating Buffer blocking buffer
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Product Notes
The Antibody Coating Buffer (Catalog #AAA258401) is a Blocking Buffer and is intended for research purposes only. The product is available for immediate purchase. 1. Dilute 5X Antibody Coating Buffer 1:5 with deionized water and mix for 15 minutes. As Antibody Coating Buffer is a 5X concentrate, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm the buffer until all crystals are dissolved. Do not let it boil. 2. Dilute your antibody into the coating buffer. Optimal coating concentration varies significantly from 0.1 ug/mL to 10 ug/mL. 3. Let the solution stir 10-15 minutes and pipette onto the plate. Optimal coating volume generally ranges between 50-200 uL per well. ICT recommends coating antibodies onto Costar 96-Well EIA/RIA Stripwell plate. 4. Once added to the plate, incubate the coating solution from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering. 5. Aspirate the coating solution. 6. Wash each well twice with 1X ELISA Wash Buffer. 7. Block the uncoated regions of the microplate wells by pipetting 300 muL of blocking buffer into each well. 8. Incubate 8-24 hours at room temperature. 9. Aspirate the blocking buffer. 10. The assay can be run at this point, or the plate can be dried and packaged for later use. 11. Dry the plate by letting it sit on the bench top from 8-24 hours while protected from light, or dry in a drying chamber under vacuum from 3-6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant packet and store at RT or 2-8 degree C protected from light. Researchers should empirically determine the suitability of the Antibody Coating Buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Antibody Coating Buffer, Blocking Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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