BLK recombinant protein

BLK, 1-505aa, Human, His tag, Insect cell

Cat. Num. AAA204484

• Gene Names: BLK; MODY11
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: BLK; 1-505aa; Human; His tag; Insect cell; Tyrosine-protein kinase Blk; MGC10442; MODY11; BLK recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 7.5) containing 50% glycerol, 0.25M NaCl, 5mM DTT, 0.1mM PMSF, 0.1mM EDTA
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ YELQP
• Sequence Length: 505

• Applicable Applications for BLK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BLK recombinant protein

BLK, also known as B lymphoid kinase, is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. This protein is expressed specifically in the B cell lineage and plays a role in the signaltransduction pathway that is restricted to B lymphoid cells. Recombinant human BLK protein was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.

Product Categories/Family for BLK recombinant protein

Apoptosis

References

Dymecki. et al. (1990) Science. 247:332-6.; Tatosyan, A.G. et al. (2000) Biochemistry. 65:49-58

NCBI and Uniprot Product Information

• NCBI GI #: 33469982
• NCBI GeneID: 640
• NCBI Accession #: NP_001706
• NCBI GenBank Nucleotide #: NM_001715.2
• UniProt Accession #: P51451; Q16291; Q96IN1
• Molecular Weight: 59.8 kDa (525aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: tyrosine-protein kinase Blk isoform 1
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BLK

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207'. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.