BLK / B Lymphocyte Kinase Recombinant Protein | BLK recombinant protein

Recombinant Human BLK / B Lymphocyte Kinase Protein (GST tag)

Cat. Num. AAA2545081

• Gene Names: BLK; MODY11
• Purity: > 88 % as determined by SDS-PAGE
• Synonyms: BLK / B Lymphocyte Kinase; Recombinant Human BLK / B Lymphocyte Kinase Protein (GST tag); MGC10442; MODY11; BLK recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 88 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 5mM GSH, pH 7.4
• Sequence Length: 505

• Application Notes: The recombinant human BLK/GST chimera consists of 730 amino acids and migrates as an approximatly 84 kDa band as predicted in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for BLK recombinant protein

Background: Tyrosine-protein kinase Blk, also known as B lymphocyte kinase, p55-Blk and BLK, is a member of the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. BLK / p55-Blk is expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles. BLK / p55-Blk is a src-family protein tyrosine kinase specifically expressed in B-lineage cells of mice. The early onset of Blk expression during B-cell development in the bone marrow and the high expression levels of Blk in mature B cells suggest a possible important role of Blk in B-cell physiology. It is a modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Defects in BLK are a cause of maturity-onset diabetes of the young type 11 which is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

Description: A DNA sequence encoding the human BLK (NP_001706.2) (Met 1-Pro 505) was fused with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 33469982
• NCBI GeneID: 640
• NCBI Accession #: NP_001706.2
• NCBI GenBank Nucleotide #: NM_001715.2
• UniProt Accession #: P51451; Q16291; Q96IN1
• Molecular Weight: 57,706 Da
• NCBI Official Full Name: tyrosine-protein kinase Blk isoform 1
• NCBI Official Synonym Full Names: BLK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: BLK
• NCBI Official Synonym Symbols: MODY11
• NCBI Protein Information: tyrosine-protein kinase Blk
• UniProt Protein Name: Tyrosine-protein kinase Blk
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BLK

NCBI Description

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Uniprot Description

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Src family; TK group

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: B cell receptor signaling pathway; cell differentiation; cytoskeleton organization and biogenesis; innate immune response; negative regulation of apoptosis; positive regulation of insulin secretion; regulation of cell proliferation; regulation of phagocytosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Maturity-onset Diabetes Of The Young, Type 11