Structure

Phosphate tris(acetoxymethyl)ester (PO4-AM3) Biochemical

Phosphate tris(acetoxymethyl)ester (PO4-AM3)

Purity

Typical analysis is better than 95% (MS). The product is not sterile and has not been tested for endotoxins

Synonyms

Phosphate tris(acetoxymethyl)ester (PO4-AM3); Phosphate tris(acetoxymethyl)ester (PO4-AM3) biochemical

Ordering
Cat. Num. AAA256162 For Research Use Only!

Specificity

Micromolar quantities are determined by weight.0.33 ?mol of PO4-AM3 is stoichiometrically equivalentto 1 ?mol of a cyclic nucleotide-AM este.

Purity/Purification

Typical analysis is better than 95% (MS). The product is not sterile and has not been tested for endotoxins

Properties

PO4-AM3is a membrane- permeable prodrug of inorganic phosphate. The acetoxymethyl group s mask the charge d polar phosphate and thus render the molecule highly membrane - permeant. Inside the cell esterases release the highly polar inorganic phosphate (PO 4 3 - ) . In addition, the AM ester groups liberate acetic aci d and formaldehyde, two metabolites with potential biological functions. PO 4 - AM 3 is recommended as control reagent in nucleotide - AM ester applications to test for side effects of acetic acid and formaldehyde.

Solubility

Due to its rather high lipophilicity, the solubility of PO4-AM3in water or buffers is limited. We suggest to use a small amount of anhydrous organic solvent such as anhydrous DMSO or DMF for preparation of 1-100 mM stock solutions, and to dilute withwater or buffer down to the concentrations required. In some cases, especially at high concentrations (~ 1mM), Pluronic?F-127 (Molecular Probes) can be useful to facilitate solubilization in water or physiological media. Please be sure to check for DMSO/DMF tolerance in your system. Since PO4-AM3is bioactivated by esterases, application to cell cultures should be performed without serum supplements (even heat-inactivated serum still contains active esterases!) in the media for at least 15 minutes. Otherwise, serum esterases may strongly reduce the cell-loading efficacy. Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.

Formula

C₉H₁₅O₁₀P

CAS No

[148334-61-6]

Preparation and Storage

PO4-AM3is sufficiently stable to be shipped at ambient temperature, however, it should be stored in the freezer (-20°Cnecessary, -80°C recommended).Please note that aqueous solutions are rather labile and should be freshly prepared immediately before use. Stock solutions in anhydrous DMSO or DMF shouldbe relatively stable when stored frozen at -20°C to -80°C.

8- (2- [ Fluoresceinyl]aminoethylthio)adenosine- 3', 5'- cyclic monophosphate (8-[Fluo]-cAMP / 8-[Fluo]-AET-cAMP) Biochemical

Host: n/a

Reactivity: n/a

Apps: n/a

Product Notes

The Phosphate tris(acetoxymethyl)ester (PO4-AM3) (Catalog #AAA256162) is a Biochemical and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Phosphate tris(acetoxymethyl)ester (PO4-AM3), Biochemical" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Loading...

Phosphate tris(acetoxymethyl)ester (PO4-AM3) Biochemical