BH3 Interacting Domain Death Agonist Recombinant Protein | BID recombinant protein

Recombinant Human BH3 Interacting Domain Death Agonist

Cat. Num. AAA143405

• Gene Names: BID; FP497
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: BH3 Interacting Domain Death Agonist; Recombinant Human BH3 Interacting Domain Death Agonist; BID Human; BH3 Interacting Domain Death Agonist Human Recombinant; BH3-interacting domain death agonist; p22 BID; BID; FP497; MGC15319; MGC42355; BID recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The protein solution contains 20mM Tris-HCl pH-8 & 20% NaCl.; Sterile Filtered colorless solution.
• Sequence: MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ WEGYDELQTD GNRSSHSRLG RIEADSESQE DIIRNIARHL AQVGDSMDRS IPPGLVNGLA LQLRNTSRSE EDRNRDLATA LEQLLQAYPR DMEKEKTMLV LALLLAKKVA SHTPSLLRDV FHTTVNFINQ NLRTYVRSLA RNGMD
• Sequence Length: 195

Related Product Information for BID recombinant protein

Description: BID Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids and having a molecular mass of 21.9 kDa.

Introduction: BID accession number NP_001187 is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.

Product Categories/Family for BID recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; BID

NCBI and Uniprot Product Information

• NCBI GI #: 4557361
• NCBI GeneID: 637
• NCBI Accession #: NP_001187.1
• NCBI GenBank Nucleotide #: NM_001196.3
• UniProt Accession #: P55957; Q549M7; Q71T04; Q7Z4M9; Q8IY86
• Molecular Weight: 11,263 Da
• NCBI Official Full Name: BH3-interacting domain death agonist isoform 2
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: BID
• NCBI Official Synonym Symbols: FP497
• NCBI Protein Information: BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; Human BID coding sequence; apoptic death agonist; desmocollin type 4; p22 BID
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: BH3-interacting domain death agonist
• UniProt Gene Name: BID
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_HUMAN

NCBI Description

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; positive regulation of protein homooligomerization; apoptosis; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; neuron apoptosis; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization