Benzonase Nuclease Recombinant Protein
Recombinant Serratia Marcescens Benzonase Nuclease
Purity
Greater than 99.0% as determined by SDS-PAGE.
Synonyms
Benzonase Nuclease; Recombinant Serratia Marcescens Benzonase Nuclease; Benzonase Nuclease Serratia Marcescens Recombinant; Welqut Protease Staphylococcus aureus Recombinant; Benzonase Nuclease recombinant protein
Host
E Coli
Specificity
Unspecific (DNA, RNA) attacks all nucleic acids (single strand, double strand, circular, supercoiled) with no apparent sequence preference. Final reaction product:
5’-mono-phosphate terminated oligonucleotides (3-5 bases).
Protease Activity: Not detectable
5’-mono-phosphate terminated oligonucleotides (3-5 bases).
Protease Activity: Not detectable
Purity/Purification
Greater than 99.0% as determined by SDS-PAGE.
Form/Format
Sterile Filtered colorless solution.The Benzonase Nuclease (250U/ul) solution contains 50% glycerol, 50 mM Tris-HCl pH 8.0, 20 mM NaCl and 2 mM MgCl2.
Unit Definition
1U Benzonase Nuclease is defined as the amount of enzyme that causes a deltaA260 of 1 in 30 min, which corresponds to complete digestion of 37ug DNA. Standard reaction conditions are 1mg/ml sonicated DNA substrate in 50mM Tris-HCl pH 8.0, 0.1mg/ml BSA, 1mM MgCl2, incubated at 37 degree C.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Related Product Information for Benzonase Nuclease recombinant protein
Introduction: Serratia marcescens secretes an endonuclease that has exceptionally high specific activity to the medium that surrounds it. The Benzonase Nuclease is mainly used for elimination of nucleic acid contamination from purified proteins, downstream processing, reduction of viscosity etc. Nucleic acid contaminants are caused by nuclease released to the medium. The DNA is being destroyed by the release of the S. marcescens nuclease and it acts as the killer gene for the auto destruction of microorganisms.
Description: Benzonase Nuclease Serratia Marcescens Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 245 amino acids and having a molecular mass of 30kDa with 2 essential disulfide bonds.
Benzonase Nuclease is purified by proprietary chromatographic techniques.
Description: Benzonase Nuclease Serratia Marcescens Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 245 amino acids and having a molecular mass of 30kDa with 2 essential disulfide bonds.
Benzonase Nuclease is purified by proprietary chromatographic techniques.
Product Categories/Family for Benzonase Nuclease recombinant protein
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Product Notes
The Benzonase Nuclease (Catalog #AAA141490) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Benzonase Nuclease, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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