Testing Data
(P-peptide-+)
HSP20 Antibody | anti-HSP20 antibody
HSP20 (Phospho-Ser16) Antibody
Gene Names
HSPB6; HEL55; Hsp20; PPP1R91
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
HSP20; HSP20 (Phospho-Ser16) Antibody; HSPB6; Heat-shock 20 kDa like-protein p20; anti-HSP20 antibody
Reactivity
Human, Mouse, Rat
Specificity
HSP20 (Phospho-Ser16) antibody detects endogenous levels of HSP20 only when phosphorylated at serine 16.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
160
Applicable Applications for anti-HSP20 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
IF: 1:100-1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human HSP20 around the phosphorylation site of serine 16 (R-A-SP-A-P).
All Sites
Human: Ser16
Mouse: Ser16
Rat: Ser16
Mouse: Ser16
Rat: Ser16
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HSP20 (Phospho-Ser16) antibody. Immunofluorescence analysis of HeLa cells, using HSP20 (Phospho-Ser16) antibody.)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HSP20 (Phospho-Ser16) antibody. Immunofluorescence analysis of HeLa cells, using HSP20 (Phospho-Ser16) antibody.)
References
Guo-Chang Fan, Circ. Res., Jun 2004; 94: 1474-1482. Junzo Norimine, Infect. Immun., Feb 2004; 72: 1096-1106. Marco Ventura, Appl. Envir. Microbiol., Jul 2007; 73: 4695-4703. Charles R. Flynn, FASEB J, May 2003; 10.1096/fj.02-1028fje.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,136 Da
NCBI Official Full Name
heat shock protein beta-6
NCBI Official Synonym Full Names
heat shock protein family B (small) member 6
NCBI Official Symbol
HSPB6
NCBI Official Synonym Symbols
HEL55; Hsp20; PPP1R91
NCBI Protein Information
heat shock protein beta-6
UniProt Protein Name
Heat shock protein beta-6
Protein Family
UniProt Gene Name
HSPB6
UniProt Synonym Gene Names
HspB6
NCBI Description
This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. [provided by RefSeq, Jan 2012]
Uniprot Description
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity.
Research Articles on HSP20
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Product Notes
The HSP20 hspb6 (Catalog #AAA8509456) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The HSP20 (Phospho-Ser16) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP20 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50-1:100 IF: 1:100-1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the HSP20 hspb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP20, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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