BCCIP recombinant protein

BCCIP Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121689

• Gene Names: BCCIP; TOK1; TOK-1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: BCCIP; BCCIP Protein; Human; Recombinant (His & GST Tag); Human BRCA2/BCCIP Protein (His & GST Tag); TOK-1 Protein; TOK1 Protein; BRCA2 and CDKN1A interacting protein; BCCIP recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val314

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human BCCIP (AAH09771.1)(Met1-Val314) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BCCIP recombinant protein

Background: BCCIP was originally identified as a BRCA2 and CDKN1A interacting protein that has been implicated in maintenance of genomic stability, cell cycle regulation, and microtubule dynamics. The secondary genetic alternations may overcome the progression suppression imposed by BCCIP deficiency through a synthetic viability mechanism. Knockdown of YY1 inhibited the binding of BCCIP itself at BCCIP promoter region proximal to TSS, demonstrating that transcriptional regulation of the YY1 on BCCIP can be modulated by BCCIP itself in a YY1-dependent fashion. BCCIP (BRCA2 and CDKN1A interacting protein) plays a critical role in maintaining the critical functions of p53 in tumor suppression and response to therapy. Celecoxib affects the functions of p53 and inhibits the recovery from the irradiation-induced injury by up-regulating the expression of BCCIP, and subsequently regulates the expressions of genes such as p21 and Cyclin B1 to enhance the radiosensitivity of HCT116 cells in a COX-2 independent manner.

NCBI and Uniprot Product Information

• NCBI GI #: 14602523
• NCBI GeneID: 56647
• Molecular Weight: 33,242 Da
• NCBI Official Full Name: BRCA2 and CDKN1A interacting protein
• NCBI Official Synonym Full Names: BRCA2 and CDKN1A interacting protein
• NCBI Official Symbol: BCCIP
• NCBI Official Synonym Symbols: TOK1; TOK-1
• NCBI Protein Information: BRCA2 and CDKN1A-interacting protein; BCCIPalpha; BCCIPbeta; BRCA2 and Cip1/p21 interacting protein; TOK-1alpha; TOK-1beta; cdk inhibitor p21 binding protein; p21- and CDK-associated protein 1; protein TOK-1
• Protein Family: BRCA2 and CDKN1A-interacting protein

NCBI Description

This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]