Human Brevican ELISA Kit | BCAN elisa kit

Human Brevican PicoKine ELISA Kit

Cat. Num. AAA1752626

• Gene Names: BCAN; BEHAB; CSPG7
• Reactivity: Human
• Synonyms: Brevican; Human Brevican PicoKine ELISA Kit; Human Brevican; brevican; Brevican core protein; Brain-enriched hyaluronan-binding protein; BEHAB; Chondroitin sulfate proteoglycan 7; BCAN; CSPG7; UNQ2525; PRO6018; BCAN elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human BCAN
• Sequence Length: 911

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCAN elisa kit

Principle of the Assay: The Picokine™ Human BCAN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human BCAN with a 96-well strip plate that is pre-coated with antibody specific for BCAN. The detection antibody is a biotinylated antibody specific for BCAN. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human BCAN with immunogen: Expression system for standard: NSO, Immunogen sequence: D23-P911. The kit is analytically validated with ready to use reagents. To measure Human BCAN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human BCAN in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human BCAN in the sample.!!Background/Introduction: Brevican core protein is a protein that in humans is encoded by the BCAN gene. This gene is mapped to 1q23.1. Brevican is a member of the lectican protein family. Brevican is localised to the surface of neurons in the brain. In melanocytic cells, BCAN gene expression may be regulated by MITF. This gene encodes a member of the lectican family of chondroitin sulfate proteoglycans that is specifically expressed in the central nervous system. This protein is developmentally regulated and may function in the formation of the brain extracellular matrix. This protein is highly expressed in gliomas and may promote the growth and cell motility of brain tumor cells. Alternate splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 38372935
• NCBI GeneID: 63827
• NCBI Accession #: NP_068767.3
• NCBI GenBank Nucleotide #: NP_068767.3
• UniProt Accession #: Q96GW7
• NCBI Official Full Name: brevican core protein isoform 1
• NCBI Official Synonym Full Names: brevican
• NCBI Official Symbol: BCAN
• NCBI Official Synonym Symbols: BEHAB; CSPG7
• NCBI Protein Information: brevican core protein
• UniProt Protein Name: Brevican core protein
• Protein Family: Brevican core protein
• UniProt Gene Name: BCAN
• UniProt Synonym Gene Names: BEHAB; CSPG7; BEHAB
• UniProt Entry Name: PGCB_HUMAN

NCBI Description

This gene encodes a member of the lectican family of chondroitin sulfate proteoglycans that is specifically expressed in the central nervous system. This protein is developmentally regulated and may function in the formation of the brain extracellular matrix. This protein is highly expressed in gliomas and may promote the growth and cell motility of brain tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

BCAN: May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. Belongs to the aggrecan/versican proteoglycan family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Membrane protein, GPI anchor; Secreted

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: chondroitin sulfate metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; central nervous system development; glycosaminoglycan metabolic process; chondroitin sulfate catabolic process; hippocampus development; carbohydrate metabolic process; pathogenesis; cell adhesion; skeletal development; dermatan sulfate biosynthetic process