Human Bcl 2 Antagonist/Killer 1 ELISA Kit | BAK1 elisa kit

Human Bcl 2 Antagonist/Killer 1 ELISA Kit

Cat. Num. AAA31693

• Gene Names: BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
• Reactivity: Human
• Synonyms: Bcl 2 Antagonist/Killer 1; Human Bcl 2 Antagonist/Killer 1 ELISA Kit; BAK1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human BAK1 and analogues was observed.
• Sequence Length: 211

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated BAK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAK1 and BAK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for BAK1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated BAK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAK1 and BAK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502363
• NCBI GeneID: 578
• NCBI Accession #: NP_001179.1
• NCBI GenBank Nucleotide #: NM_001188.3
• UniProt Accession #: Q16611; Q6I9T6; Q92533
• Molecular Weight: 23,409 Da
• NCBI Official Full Name: bcl-2 homologous antagonist/killer
• NCBI Official Synonym Full Names: BCL2-antagonist/killer 1
• NCBI Official Symbol: BAK1
• NCBI Official Synonym Symbols: BAK; CDN1; BCL2L7; BAK-LIKE
• NCBI Protein Information: bcl-2 homologous antagonist/killer; bcl2-L-7; BCL2-like 7 protein; bcl-2-like protein 7; apoptosis regulator BAK; pro-apoptotic protein BAK
• UniProt Protein Name: Bcl-2 homologous antagonist/killer
• Protein Family: Bcl-2 homologous antagonist/killer
• UniProt Gene Name: BAK1
• UniProt Synonym Gene Names: BAK; BCL2L7; CDN1; Bcl2-L-7
• UniProt Entry Name: BAK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]

Uniprot Description

BAK1: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. Interacts with BCL2A1. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). Interacts with myxoma virus protein M11L. Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. Belongs to the Bcl-2 family.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol; integral to mitochondrial outer membrane

Molecular Function: BH domain binding; identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; chaperone binding; metal ion binding

Biological Process: response to fungus; regulation of protein heterodimerization activity; positive regulation of proteolysis; regulation of cell cycle; apoptosis; positive regulation of apoptosis; myeloid cell homeostasis; negative regulation of peptidyl-serine phosphorylation; B cell apoptosis; response to organic cyclic substance; negative regulation of cell proliferation; regulation of mitochondrial membrane potential; B cell homeostasis; response to gamma radiation; establishment and/or maintenance of transmembrane electrochemical gradient; B cell negative selection; aging; response to drug; organ regeneration; release of cytochrome c from mitochondria; response to mycotoxin; mitochondrial fusion; regulation of protein homodimerization activity; vagina development; limb morphogenesis; endocrine pancreas development; response to UV-C; cell proliferation; endoplasmic reticulum calcium ion homeostasis; unfolded protein response, activation of signaling protein activity; response to ethanol; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; brain development; regulation of mitochondrial membrane permeability; caspase activation via cytochrome c; post-embryonic camera-type eye morphogenesis