EGFL7 lysate

Human EGFL7 Baculovirus-Insect cells Overexpression Lysate

Cat. Num. AAA8119635

• Applications: Western Blot
• Synonyms: EGFL7; Human EGFL7 Baculovirus-Insect cells Overexpression Lysate; Human EGFL7/VE-statin Insect Cell Lysate (WB positive control); Human NEU1 Overexpression Lysate; Human VE-STATIN Overexpression Lysate; Human ZNEU1 Overexpression Lysate; EGF-like-domain; multiple 7; EGFL7 lysate

• Host: Baculovirus-Insect cells
• Form/Format: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.; 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

• Applicable Applications for EGFL7 lysate: Western Blot (WB)
• Application Notes: Western blot (WB): Use at an assay dependent dilution.
• Species: Human
• Sequence Information: A DNA sequence encoding the mature form of human EGFL7 (NP_057299.1)(Tyr 24-Ser 273) was expressed, with a polyhistidine tag at the N-terminus.
• Preparation Method: Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Recommend Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.; 2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
• Preparation and Storage: Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.

Related Product Information for EGFL7 lysate

Description: This Human EGFL7 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of EGFL7 protein from the overexpression lysate was verified.

Background: Baculovirus-Insect Cell lysate that Human EGFL7/MEGF7 transfected/overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains twoEGF-like domains and oneEMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.

References

Fitch,M.J. et al., 2004, Dev Dyn. 230 (2):316-24. Schmidt,M. et al., 2007, Novartis Found Symp. 283 :18-28. Campagnolo,L.et al., 2008, Gene Expr Patterns 8 (6):389-96. Picuric,S. et al., 2009, Protein Expr Purif. 68 (1):1-6. Nikolic,I. et al., 2010, J Angiogenes Res. 2 (1): 9.