GLT25D2 Cell Lysate | COLGALT2 cell lysate
Human GLT25D2 Baculovirus-Insect cells Overexpression Lysate
Gene Names
COLGALT2; C1orf17; GLT25D2
Applications
Western Blot
Synonyms
GLT25D2; Human GLT25D2 Baculovirus-Insect cells Overexpression Lysate; Human GLT25D2 Insect Cell Lysate (WB positive control); collagen beta(1-O)galactosyltransferase 2; Human C1orf17 Overexpression Lysate; Human GLT25D2 Overexpression Lysate; Human RP11-498P10.2 Overexpression Lysate; COLGALT2 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
632aa
Sequence
Met1-Ser622
Applicable Applications for COLGALT2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human GLT25D2 (Q8IYK4) (Met 1-Ser 622) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for COLGALT2 cell lysate
Glycosyl transferase 25 domain 2 (GLT25D2) is a glucosyltransferase enzyme expressed only at low levels in the nervous system. Glycosyltransferases are enzymes that act as a catalyst for the transfer of a monosaccharide unit from an activated nucleotide sugar (also known as the "glycosyl donor") to a glycosyl acceptor molecule, usually an alcohol. Glycosyl transferases transfer glycosyl groups onto their substrate. Localization partially defines their function. Glt25D2 enzyme showed a strong galactosyltransferase activity toward various types of collagen and toward the serum mannose-binding lectin MBL which contains a collagen domain.
This Human GLT25D2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of GLT25D2 protein from the overexpression lysate was verified.
This Human GLT25D2 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of GLT25D2 protein from the overexpression lysate was verified.
References
Schegg B, et al. (2009) Core glycosylation of collagen is initiated by two beta (1-O) galactosyltransferases. Mol Cell Biol. 29 (4): 943-52.Sricholpech M, et al. (2011) Lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture. J Biol Chem. 286 (11): 8846-56.O'Connor DH, et al. (2009) Reverse engineering the mouse brain. Nature. 461 (7266): 923-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,924 Da
NCBI Official Full Name
procollagen galactosyltransferase 2
NCBI Official Synonym Full Names
collagen beta(1-O)galactosyltransferase 2
NCBI Official Symbol
COLGALT2
NCBI Official Synonym Symbols
C1orf17; GLT25D2
NCBI Protein Information
procollagen galactosyltransferase 2; hydroxylysine galactosyltransferase 2; glycosyltransferase 25 family member 2; glycosyltransferase 25 domain containing 2
UniProt Protein Name
Procollagen galactosyltransferase 2
Protein Family
UniProt Gene Name
COLGALT2
UniProt Synonym Gene Names
C1orf17; GLT25D2; KIAA0584
UniProt Entry Name
GT252_HUMAN
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Product Notes
The COLGALT2 colgalt2 (Catalog #AAA8114972) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 632aa. AAA Biotech's GLT25D2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the COLGALT2 colgalt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser62 2. It is sometimes possible for the material contained within the vial of "GLT25D2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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