SDS-PAGE
GLT25D2 recombinant protein
Recombinant Human GLT25D2 Protein (His tag)
Gene Names
COLGALT2; C1orf17; GLT25D2
Purity
> 85 % as determined by SDS-PAGE
Synonyms
GLT25D2; Recombinant Human GLT25D2 Protein (His tag); RP11-498P10.2; C1orf17; FLJ37771; FLJ37873; KIAA0584; GLT25D2 recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
> 85 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4
Sequence Length
554
Application Notes
The recombinant human GLT25D2 consists of 632 amino acids and predicts a molecular mass of 73.8 kDa. It migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Met 1
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for GLT25D2 recombinant protein
Background: Glycosyl transferase 25 domain 2 (GLT25D2) is a glucosyltransferase enzyme expressed only at low levels in the nervous system. Glycosyltransferases are enzymes that act as a catalyst for the transfer of a monosaccharide unit from an activated nucleotide sugar (also known as the "glycosyl donor") to a glycosyl acceptor molecule, usually an alcohol. Glycosyl transferases transfer glycosyl groups onto their substrate. Localization partially defines their function. Glt25D2 enzyme showed a strong galactosyltransferase activity toward various type s of collagen and toward the serum mannose-binding lectin MBL which contains a collagen domain.
Description: A DNA sequence encoding the human GLT25D2 (Q8IYK4) (Met 1-Ser 622) was fused with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the human GLT25D2 (Q8IYK4) (Met 1-Ser 622) was fused with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,924 Da
NCBI Official Full Name
procollagen galactosyltransferase 2 isoform 2
NCBI Official Synonym Full Names
collagen beta(1-O)galactosyltransferase 2
NCBI Official Symbol
COLGALT2
NCBI Official Synonym Symbols
C1orf17; GLT25D2
NCBI Protein Information
procollagen galactosyltransferase 2
UniProt Protein Name
Procollagen galactosyltransferase 2
UniProt Gene Name
COLGALT2
UniProt Synonym Gene Names
C1orf17; GLT25D2; KIAA0584
Uniprot Description
GLT25D2: Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues on collagen. Belongs to the glycosyltransferase 25 family.
Protein type: EC 2.4.1.50; Transferase
Chromosomal Location of Human Ortholog: 1q25.3
Cellular Component: endoplasmic reticulum lumen
Molecular Function: procollagen galactosyltransferase activity; protein binding
Research Articles on GLT25D2
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Product Notes
The GLT25D2 colgalt2 (Catalog #AAA2545490) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human GLT25D2 consists of 632 amino acids and predicts a molecular mass of 73.8 kDa. It migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the GLT25D2 colgalt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLT25D2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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