Lymphotactin/XCL1 Cell Lysate | XCL1 cell lysate
Mouse Lymphotactin/XCL1 Baculovirus-Insect cells Overexpression Lysate
Gene Names
Xcl1; LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
Applications
Western Blot
Synonyms
Lymphotactin/XCL1; Mouse Lymphotactin/XCL1 Baculovirus-Insect cells Overexpression Lysate; Mouse XCL1 Insect Cell Lysate (WB positive control); chemokine (C motif) ligand 1; Mouse AI661682 Overexpression Lysate; Mouse ATAC Overexpression Lysate; Mouse Lptn Overexpression Lysate; Mouse LTN Overexpression Lysate; Mouse SCM-1 Overexpression Lysate; Mouse SCM-1a Overexpression Lysate; Mouse Scyc1 Overexpression Lysate; XCL1 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
103aa
Sequence
Met1-Gly114
Applicable Applications for XCL1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse XCL1 (P47993) (Met 1-Gly 114) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for XCL1 cell lysate
This antimicrobial gene encodes a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation. The encoded protein is a member of the C-chemokine subfamily, retaining only two of four cysteines conserved in other chemokines, and is thought to be specifically chemotactic for T cells. This gene and a closely related family member are located on the long arm of chromosome 1.
This Mouse Lymphotactin/XCL1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Lymphotactin/XCL1 protein from the overexpression lysate was verified.
This Mouse Lymphotactin/XCL1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Lymphotactin/XCL1 protein from the overexpression lysate was verified.
References
Nguyen KD, et al. (2008) XCL1 enhances regulatory activities of CD4+ CD25(high) CD127(low/-) T cells in human allergic asthma. J Immunol. 181 (8): 5386-95.
Dong C, et al. (2005) Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity. J Immunol Methods. 302 (1-2): 136-44.
Zavala-Flores LM, et al. (2009) Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis. Biotechnol Lett. 31 (2): 215-20.
Guzzo C, Fox JC, Miao H, Volkman BF, Lusso P. Structural Determinants for the Selective Anti-HIV-1 Activity of the All-beta Alternative Conformer of XCL1. Kirchhoff F, ed. Journal of Virology. 2015;89(17):9061-9067.
Dong C, et al. (2005) Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity. J Immunol Methods. 302 (1-2): 136-44.
Zavala-Flores LM, et al. (2009) Production of biologically active human lymphotactin (XCL1) by Lactococcus lactis. Biotechnol Lett. 31 (2): 215-20.
Guzzo C, Fox JC, Miao H, Volkman BF, Lusso P. Structural Determinants for the Selective Anti-HIV-1 Activity of the All-beta Alternative Conformer of XCL1. Kirchhoff F, ed. Journal of Virology. 2015;89(17):9061-9067.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Lymphotactin
NCBI Official Synonym Full Names
chemokine (C motif) ligand 1
NCBI Official Symbol
Xcl1
NCBI Official Synonym Symbols
LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682
NCBI Protein Information
lymphotactin
UniProt Protein Name
Lymphotactin
Protein Family
UniProt Gene Name
Xcl1
UniProt Synonym Gene Names
Lptn; Ltn; Scyc1
UniProt Entry Name
XCL1_MOUSE
Uniprot Description
XCL1: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol
Molecular Function: protein homodimerization activity; chemokine activity; chemokine receptor binding; cytokine activity
Biological Process: positive regulation of T cell mediated cytotoxicity; response to virus; negative regulation of transcription factor activity; positive regulation of T cell cytokine production; positive regulation of leukocyte chemotaxis; positive regulation of immune response to tumor cell; negative regulation of T cell cytokine production; chemotaxis; positive regulation of interleukin-10 production; negative regulation of interleukin-2 production; cell-cell signaling; release of sequestered calcium ion into cytosol; negative regulation of interferon-gamma production; regulation of inflammatory response; positive regulation of innate immune response; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of transcription, DNA-dependent
Research Articles on XCL1
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Product Notes
The XCL1 xcl1 (Catalog #AAA8116782) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 103aa. AAA Biotech's Lymphotactin/XCL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the XCL1 xcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly11 4. It is sometimes possible for the material contained within the vial of "Lymphotactin/XCL1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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