Ccl21a cell lysate
Mouse Ccl21a Baculovirus-Insect Overexpression Lysate
Gene Names
Ccl21a; ALP; SLC; plt; CKb9; Tca4; 6Ckine; Scya21; 6CKBAC2; SCYA21a; Scya21b; AW987545; MGC107632; Ccl21a
Applications
Western Blot
Synonyms
Ccl21a; Mouse Ccl21a Baculovirus-Insect Overexpression Lysate; Mouse CCL21a Insect Cells Cell Lysate (WB positive control); chemokine (C-C motif) ligand 21A (serine); Mouse 6CKBAC2 Overexpression Lysate; Mouse 6Ckine Overexpression Lysate; Mouse ALP Overexpression Lysate; Mouse CKb9 Overexpression Lysate; Mouse plt Overexpression Lysate; Mouse Scya21 Overexpression Lysate; Mouse SCYA21a Overexpression Lysate; Mouse Scya21b Overexpression Lysate; Mouse SLC Overexpression Lysate; Mouse Tca4 Overexpression Lysate; Ccl21a cell lysate
Host
Baculovirus-Insect Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
110aa
Sequence
Met1-Gly133
Applicable Applications for Ccl21a cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse CCL21A (NP_035254.1) (Met1-Gly133) was expressed.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for Ccl21a cell lysate
This Mouse Ccl21a overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of Ccl21a protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
C-C motif chemokine 21b [Mus musculus]
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 21A (serine)
NCBI Official Symbol
Ccl21a
NCBI Official Synonym Symbols
ALP; SLC; plt; CKb9; Tca4; 6Ckine; Scya21; 6CKBAC2; SCYA21a; Scya21b; AW987545; MGC107632; Ccl21a
UniProt Protein Name
C-C motif chemokine 21a
Protein Family
UniProt Gene Name
Ccl21a
UniProt Synonym Gene Names
Scya21; Scya21a; TCA4
UniProt Entry Name
CC21A_MOUSE
Uniprot Description
CCL21a: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T- cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Mice carrying an autosomal recessive mutation designated paucity of lymph node T-cells (plt) show dramatically reduced numbers of T-cells in lymph nodes, Peyer patches, and the white pulp of the spleen. Plt seems to correspond to Scya21b. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine
Cellular Component: extracellular space; intracellular
Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding
Biological Process: G-protein coupled receptor protein signaling pathway; induction of positive chemotaxis; inflammatory response; lymph node development; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity
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Product Notes
The Ccl21a ccl21a (Catalog #AAA8117183) is a Cell Lysate produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 110aa. AAA Biotech's Ccl21a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the Ccl21a ccl21a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly13 3. It is sometimes possible for the material contained within the vial of "Ccl21a, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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