CaMKI gamma/CAMK1G Cell Lysate | CAMK1G cell lysate
Human CaMKI gamma/CAMK1G Baculovirus-Insect cells Overexpression Lysate
Gene Names
CAMK1G; VWS1; CLICK3; CLICKIII; dJ272L16.1
Applications
Western Blot
Synonyms
CaMKI gamma/CAMK1G; Human CaMKI gamma/CAMK1G Baculovirus-Insect cells Overexpression Lysate; Human CAMK1G/CLICK III/CaMKIgamma Insect Cell Lysate (WB positive control); calcium/calmodulin-dependent protein kinase IG; Human CLICK3 Overexpression Lysate; Human CLICKIII Overexpression Lysate; Human dJ272L16.1 Overexpression Lysate; Human RP1-272L16.2 Overexpression Lysate; Human VWS1 Overexpression Lysate; CAMK1G cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
713aa
Sequence
Met1-Met476
Applicable Applications for CAMK1G cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CAMK1G isoform 1 (Q96NX5-1) (Met 1-Met 476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for CAMK1G cell lysate
Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIgamma is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIgamma membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIgamma critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIgamma specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIgamma may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.
This Human CaMKI gamma/CAMK1G overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CaMKI gamma/CAMK1G protein from the overexpression lysate was verified.
This Human CaMKI gamma/CAMK1G overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of CaMKI gamma/CAMK1G protein from the overexpression lysate was verified.
Product Categories/Family for CAMK1G cell lysate
References
Davare MA, et al. (2009) Transient receptor potential canonical 5 channels activate Ca2+/calmodulin kinase Igamma to promote axon formation in hippocampal neurons. J Neurosci. 29 (31): 9794-808.Takemoto-Kimura S, et al. (2007) Regulation of dendritogenesis via a lipid-raft-associated Ca2+/calmodulin-dependent protein kinase CLICK-III/CaMKIgamma. Neuron. 54 (5): 755-70.Harrill JA, et al. (2010) Splice variant specific increase in Ca2+/calmodulin-dependent protein kinase 1-gamma mRNA expression in response to acute pyrethroid exposure. J Biochem Mol Toxicol. 24 (3): 174-86.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
476
NCBI Official Full Name
Calcium/calmodulin-dependent protein kinase type 1G
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase IG
NCBI Official Symbol
CAMK1G
NCBI Official Synonym Symbols
VWS1; CLICK3; CLICKIII; dJ272L16.1
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type 1G; caMKIG; CLICK III; caMKI gamma; caM-KI gamma; caM kinase IG; caM kinase I gamma; caMK-like CREB kinase III
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type 1G
Protein Family
UniProt Gene Name
CAMK1G
UniProt Synonym Gene Names
CLICK3; VWS1; CaM kinase IG; CaM-KI gamma; CaMKI gamma; CaMKIG; CLICK III
UniProt Entry Name
KCC1G_HUMAN
NCBI Description
This gene encodes a protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known. [provided by RefSeq, Jul 2008]
Uniprot Description
CAMK1G: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.17; CAMK group; CAMK1 family
Chromosomal Location of Human Ortholog: 1q32.2
Cellular Component: Golgi membrane; neuron projection; plasma membrane; calcium- and calmodulin-dependent protein kinase complex
Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding
Biological Process: protein amino acid phosphorylation
Research Articles on CAMK1G
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Product Notes
The CAMK1G camk1g (Catalog #AAA8115055) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 713aa. AAA Biotech's CaMKI gamma/CAMK1G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the CAMK1G camk1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Met47 6. It is sometimes possible for the material contained within the vial of "CaMKI gamma/CAMK1G, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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