Calcineurin B/PPP3R1 Cell Lysate | PPP3R1 cell lysate
Human Calcineurin B/PPP3R1 Baculovirus-Insect cells Overexpression Lysate
Gene Names
PPP3R1; CNB; CNB1; CALNB1
Applications
Western Blot
Synonyms
Calcineurin B/PPP3R1; Human Calcineurin B/PPP3R1 Baculovirus-Insect cells Overexpression Lysate; Human PPP3R1 Insect Cell Lysate (WB positive control); protein phosphatase 3; regulatory subunit B; alpha; Human CALNB1 Overexpression Lysate; Human CNB Overexpression Lysate; Human CNB1 Overexpression Lysate; PPP3R1 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
187aa
Sequence
Gly2-Val170
Applicable Applications for PPP3R1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human PPP3R1 (P63098) (Gly2-Val170) was fused with a polyhistide tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PPP3R1 cell lysate
PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation, NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway; mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+)-pilocarpine, 2, 4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 plays an important role in the T cell activation pathway.
This Human Calcineurin B/PPP3R1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Calcineurin B/PPP3R1 protein from the overexpression lysate was verified.
This Human Calcineurin B/PPP3R1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Calcineurin B/PPP3R1 protein from the overexpression lysate was verified.
Product Categories/Family for PPP3R1 cell lysate
References
Feng B, et al. (1999) Interactions of calcineurin A, calcineurin B, and Ca2+. Biochemistry 38 (38): 12481-9.Kawamura A, et al. (1995) Interaction of FKBP12-FK506 with calcineurin A at the B subunit-binding domain. J Biol Chem. 270(26):15463-6.Wang MG, et al. (1997) Calcineurin A alpha (PPP3CA), calcineurin A beta (PPP3CB) and calcineurin B (PPP3R1) are located on human chromosomes 4, 10q21q22 and 2p16p15 respectively. Cytogenet Cell Genet. 72(2-3):236-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
170
NCBI Official Full Name
Calcineurin subunit B type 1
NCBI Official Synonym Full Names
protein phosphatase 3, regulatory subunit B, alpha
NCBI Official Symbol
PPP3R1
NCBI Official Synonym Symbols
CNB; CNB1; CALNB1
NCBI Protein Information
calcineurin subunit B type 1; calcineurin B, type I (19kDa); protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; protein phosphatase 3 (f
UniProt Protein Name
Calcineurin subunit B type 1
UniProt Gene Name
PPP3R1
UniProt Synonym Gene Names
CNA2; CNB
UniProt Entry Name
CANB1_HUMAN
Uniprot Description
PPP3R1: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Belongs to the calcineurin regulatory subunit family.
Protein type: Protein phosphatase, regulatory subunit; Cell development/differentiation; Apoptosis; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2p15
Cellular Component: nucleoplasm; cytosol; calcineurin complex; sarcolemma
Molecular Function: calmodulin binding; protein domain specific binding; protein binding; calcium-dependent protein serine/threonine phosphatase activity; calcium ion binding
Biological Process: calcineurin-NFAT signaling pathway; positive regulation of NFAT protein import into nucleus; dephosphorylation; apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter
Research Articles on PPP3R1
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Product Notes
The PPP3R1 ppp3r1 (Catalog #AAA8115241) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 187aa. AAA Biotech's Calcineurin B/PPP3R1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PPP3R1 ppp3r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gly2-Val17 0. It is sometimes possible for the material contained within the vial of "Calcineurin B/PPP3R1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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