BBOX1 cell lysate
Human BBOX1 Baculovirus-Insect cells Overexpression Lysate
Gene Names
BBOX1; BBH; BBOX; G-BBH; gamma-BBH
Applications
Western Blot
Synonyms
BBOX1; Human BBOX1 Baculovirus-Insect cells Overexpression Lysate; Human BBOX1/Gamma-BBH Insect Cell Lysate (WB positive control); butyrobetaine (gamma); 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1; Human BBH Overexpression Lysate; Human BBOX Overexpression Lysate; Human G-BBH Overexpression Lysate; Human gamma-BBH Overexpression Lysate; BBOX1 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
624aa
Sequence
Met1-Asn387
Applicable Applications for BBOX1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human BBOX1 (O75936) (Met1-Asn387) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for BBOX1 cell lysate
BBOX1, also known as gamma-BBH, belongs to thegamma-BBH/TMLD family. It is highly expressed in kidney and moderately expressed in liver. BBOX1 catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. BBOX1 is an inhibition target for mildronate which can be used to treatanginaandmyocardial infarction. Mildronate may also be beneficial for the treatment ofneurological disorder, diabetes, andseizures andalcohol intoxication.
This Human BBOX1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of BBOX1 protein from the overexpression lysate was verified.
This Human BBOX1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of BBOX1 protein from the overexpression lysate was verified.
References
Vaz FM. et al., 1998, Biochem Biophys Res Commun. 250 (2): 506-10. Paul HS. et al., 1992, Eur J Biochem. 203 (3): 599-605. Dawany NB. et al., 2011, Int J Cancer. 128 (12): 2881-91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
387
NCBI Official Full Name
Gamma-butyrobetaine dioxygenase
NCBI Official Synonym Full Names
butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1
NCBI Official Symbol
BBOX1
NCBI Official Synonym Symbols
BBH; BBOX; G-BBH; gamma-BBH
NCBI Protein Information
gamma-butyrobetaine dioxygenase; gamma-butyrobetaine hydroxylase; gamma-butyrobetaine,2-oxoglutarate dioxygenase 1
UniProt Protein Name
Gamma-butyrobetaine dioxygenase
Protein Family
UniProt Gene Name
BBOX1
UniProt Synonym Gene Names
BBH; BBOX; Gamma-BBH
UniProt Entry Name
BODG_HUMAN
NCBI Description
This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008]
Uniprot Description
BBOX1: Catalyzes the formation of L-carnitine from gamma- butyrobetaine. Belongs to the gamma-BBH/TMLD family.
Protein type: EC 1.14.11.1; Oxidoreductase; Amino Acid Metabolism - lysine degradation
Chromosomal Location of Human Ortholog: 11p14.2
Cellular Component: mitochondrion; cytosol
Molecular Function: gamma-butyrobetaine dioxygenase activity; zinc ion binding; iron ion binding
Biological Process: carnitine biosynthetic process
Research Articles on BBOX1
Similar Products
Product Notes
The BBOX1 bbox1 (Catalog #AAA8115538) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 624aa. AAA Biotech's BBOX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the BBOX1 bbox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn38 7. It is sometimes possible for the material contained within the vial of "BBOX1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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