GBA3 active protein

GBA3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120866

• Gene Names: GBA3; CBG; GLUC; KLRP; CBGL1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: GBA3; GBA3 Protein; Human; Recombinant (His Tag); Human GBA3/CBGL1 Protein (His Tag); CBG Protein; CBGL1 Protein; GLUC Protein; KLrP Protein; MGC104276 Protein; MGC126878 Protein; glucosidase; beta; acid 3 (gene/pseudogene); GBA3 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu469

• Species: Human
• Activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-beta-D glucopyranoside. The specific activity is >1,500pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GBA3 (NP_066024.1)(Met 1-Leu 469) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GBA3 active protein

Background: Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3/CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3/CBGL1 is present in small intestine (at protein level). GBA3/CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3/CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3/CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3/CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.

References

Yahata K., et al.,(2000), Molecular cloning and expression of a novel klotho-related protein. J. Mol. Med. 78:389-394.de Graaf M., et al., (2001), Cloning and characterization of human liver cytosolic beta-glycosidase.Biochem. J. 356:907-910.Berrin J.-G., et al.,(2002), Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the metabolism of dietary glucosides.Eur. J. Biochem. 269:249-258.

NCBI and Uniprot Product Information

• NCBI GI #: 13273313
• NCBI GeneID: 57733
• NCBI Accession #: NP_066024.1
• NCBI GenBank Nucleotide #: NM_020973.4
• UniProt Accession #: Q9H227; Q32LY7; Q3MIH4; Q53GG8; Q6NSF4; Q8NHT8; Q9H3T4; Q9H4C6
• Molecular Weight: 18,265 Da
• NCBI Official Full Name: cytosolic beta-glucosidase isoform a
• NCBI Official Synonym Full Names: glucosylceramidase beta 3 (gene/pseudogene)
• NCBI Official Symbol: GBA3
• NCBI Official Synonym Symbols: CBG; GLUC; KLRP; CBGL1
• NCBI Protein Information: cytosolic beta-glucosidase
• UniProt Protein Name: Cytosolic beta-glucosidase
• Protein Family: Cytosolic beta-glucosidase
• UniProt Gene Name: GBA3
• UniProt Synonym Gene Names: CBG; CBGL1
• UniProt Entry Name: GBA3_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

GBA3: Glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta- glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - starch and sucrose; Hydrolase; Other Amino Acids Metabolism - cyanoamino acid; EC 3.2.1.21

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cytosol

Molecular Function: beta-glucosidase activity; beta-galactosidase activity; glycosylceramidase activity

Biological Process: sphingolipid metabolic process; glycoside catabolic process; carbohydrate metabolic process; glycosylceramide catabolic process; glycosphingolipid metabolic process