FGF16 active protein

FGF16 Protein, Human, Cynomolgus, Recombinant

Cat. Num. AAA8121983

• Gene Names: FGF16; MF4; FGF-16
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FGF16; FGF16 Protein; Human; Cynomolgus; Recombinant; Human/Cynomolgus FGF16/FGF-16 Protein; fibroblast growth factor 16; FGF16 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 1 M NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg207

• Species: Human, Cynomolgus
• Activity: Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-15ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the human/cynomolgus FGF16 [(Identical to the huamn FGF16 (NP_003859.1)] (Met1-Arg207) was expressed. Human and Cynomolgus FGF16 sequences are identical.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-15 ng/ml.)

Related Product Information for FGF16 active protein

Background: Fibroblast growth factor 16 (FGF16) is preferentially expressed in the heart after birth, suggesting its regulation is associated with tissue-specific chromatin remodeling and DNA-protein interactions. Mutation of the MEF2 site resulted in a blunting of FGF16 promoter activity in transfected neonatal rat cardiac myocytes, that chromatin remodeling and MEF2 binding in the FGF16 promoter contribute to expression in the postnatal heart. FGF16 involvement in the fine tuning of the human skeleton of the hand. Impaired FGF16 function may also be responsible for connective tissue symptoms in MF4 patients. FGF16 expression is markedly increased in ovarian tumors, and FGF16 in conjunction with Wnt pathway contributes to the cancer phenotype of ovarian cells and suggests that modulation of its expression in ovarian cells might be a promising therapeutic strategy for the treatment of invasive ovarian cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 4503691
• NCBI GeneID: 8823
• NCBI Accession #: NP_003859.1
• NCBI GenBank Nucleotide #: NM_003868.2
• UniProt Accession #: O43320
• Molecular Weight: 23,759 Da
• NCBI Official Full Name: fibroblast growth factor 16
• NCBI Official Synonym Full Names: fibroblast growth factor 16
• NCBI Official Symbol: FGF16
• NCBI Official Synonym Symbols: MF4; FGF-16
• NCBI Protein Information: fibroblast growth factor 16
• UniProt Protein Name: Fibroblast growth factor 16
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF16
• UniProt Synonym Gene Names: FGF-16
• UniProt Entry Name: FGF16_HUMAN

Uniprot Description

FGF16: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation, and is required for normal cardiomyocyte proliferation and heart development. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: extracellular region; extracellular space

Molecular Function: 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: cell-cell signaling; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; metabolic process; organ morphogenesis; phosphoinositide-mediated signaling; regulation of endothelial cell proliferation; regulation of MAP kinase activity; regulation of phosphoinositide 3-kinase cascade; response to temperature stimulus; signal transduction

Disease: Metacarpal 4-5 Fusion