pIgR active protein

Recombinant human pIgR protein

Cat. Num. AAA201291

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: pIgR; Recombinant human pIgR protein; Polymeric immunoglobulin receptor; PIGR; PIgR; Poly-Ig receptor; Hepatocellular carcinoma-associated protein TB6; polymeric immunoglobulin receptor precursor; pIgR active protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 19-638aa
• Sequence: KSPIFGP EEVNSVEGNS VSITCYYPPT SVNRHTRKYW CRQGARGGCI TLISSEGYVS SKYAGRANLT NFPENGTFVV NIAQLSQDDS GRYKCGLGIN SRGLSFDVSL EVSQGPGLLN DTKVYTVDLG RTVTINCPFK TENAQKRKSL YKQIGLYPVL VIDSSGYVNP NYTGRIRLDI QGTGQLLFSV VINQLRLSDA GQYLCQAGDD SNSNKKNADL QVLKPEPELV YEDLRGSVTF HCALGPEVAN VAKFLCRQSS GENCDVVVNT LGKRAPAFEG RILLNPQDKD GSFSVVITGL RKEDAGRYLC GAHSDGQLQE GSPIQAWQLF VNEESTIPRS PTVVKGVAGG SVAVLCPYNR KESKSIKYWC LWEGAQNGRC PLLVDSEGWV KAQYEGRLSL LEEPGNGTFT VILNQLTSRD AGFYWCLTNG DTLWRTTVEI KIIEGEPNLK VPGNVTAVLG ETLKVPCHFP CKFSSYEKYW CKWNNTGCQA LPSQDEGPSK AFVNCDENSR LVSLTLNLVT RADEGWYWCG VKQGHFYGET AAVYVAVEER KAAGSRDVSL AKADAAPDEK VLDSGFREIE NKAIQDPRLF AEEKAVADTR DQADGSRASV DSGSSEEQGG SSR

• Applicable Applications for pIgR active protein: SDS-PAGE, Bioactivity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Measured by its binding ability in a functional ELISA with Human IgM. The ED50 range <=10ug/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Biological Activity

(Human IgM is coated at 2 ug/ml (100 ul/well) can bind Human pIgR. The ED50 range )

Related Product Information for pIgR active protein

pIgR, also known as polymeric immunoglobulin receptor, is responsible for transcytosis of soluble dimeric IgAs and immune. This protein binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, the cleavage occurs that separates the extracellular from the transmembrane segment. PIgR expression is under the strong regulation of cytokines, hormones, and pathogenic stimuli. The expression of pIgR is critically regulated by the pro-inflammatory cytokines, such as IL-1, IL-4, TNF-alpha, and IFN-gamma. Recombinant human pIgR, fused to Histag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for pIgR active protein

Immunology; Proteins

References

Giffroy D, et al, (2001) Scand J Immunol. 53:56-64. Kortum AN, et al, (2014) Immunogenetics. 66:267-279. Yue X, et al, (2017) Hepatolgoy. 65:1948-1962.

NCBI and Uniprot Product Information

• NCBI GI #: 31377806
• NCBI GeneID: 5284
• NCBI Accession #: NP_002635
• NCBI GenBank Nucleotide #: NM_002644
• UniProt Accession #: P01833; Q68D81; Q8IZY7
• Molecular Weight: 83,284 Da
• NCBI Official Full Name: polymeric immunoglobulin receptor
• NCBI Official Synonym Full Names: polymeric immunoglobulin receptor
• NCBI Official Symbol: PIGR
• NCBI Protein Information: polymeric immunoglobulin receptor; hepatocellular carcinoma associated protein TB6; hepatocellular carcinoma-associated protein TB6; poly-Ig receptor
• UniProt Protein Name: Polymeric immunoglobulin receptor
• Protein Family: Polymeric immunoglobulin receptor
• UniProt Gene Name: PIGR
• UniProt Synonym Gene Names: PIgR; Poly-Ig receptor

Uniprot Description

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.