BGN active protein

BGN, 38-368aa, Human, His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206825

• Gene Names: BGN; PGI; MRLS; DSPG1; PG-S1; SEMDX; SLRR1A
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: BGN; 38-368aa; Human; His-tag; Baculovirus (Bioactivity Validated); Biglycan preproprotein; DSPG1; MRLS; PG-S1; PGI; SEMDX; SLRR1A; BGN active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPDEEASGA DTSGVLDPDS VTPTYSAMCP FGCHCHLRVV QCSDLGLKSV PKEISPDTTL LDLQNNDISE LRKDDFKGLQ HLYALVLVNN KISKIHEKAF SPLRKLQKLY ISKNHLVEIP PNLPSSLVEL RIHDNRIRKV PKGVFSGLRN MNCIEMGGNP LENSGFEPGA FDGLKLNYLR ISEAKLTGIP KDLPETLNEL HLDHNKIQAI ELEDLLRYSK LYRLGLGHNQ IRMIENGSLS FLPTLRELHL DNNKLARVPS GLPDLKLLQV VYLHSNNITK VGVNDFCPMG FGVKRAYYNG ISLFNNPVPY WEVQPATFRC VTDRLAIQFG NYKKHHHHHH
• Sequence Length: 368

• Applicable Applications for BGN active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Biological Activity: Measured in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 for this effect is less or equal to 20 ug/ml.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BGN active protein

BGN, also known as biglycan preproprotein, is a small leucine-rich repeat proteoglycan (SLRP). It plays an important role in the development and maintenance of many tissues. Core protein of this protein binds to the growth factors BMP-4 and influences its bioactivity. This protein is also involved in collagen fiber assembly. Recombinant human BGN protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for BGN active protein

Signal Transduction; Cytoskeleton

References

Sun H., et al, (2016) Arch Gynecol Obstet. 293:429-438. Gaspar R., et al, (2016) J Mol Cell Cardiol. 99:138-150.

NCBI and Uniprot Product Information

• NCBI GI #: 4502403
• NCBI GeneID: 633
• NCBI Accession #: NP_001702
• NCBI GenBank Nucleotide #: NM_001711.5
• UniProt Accession #: P21810; P13247; D3DWU3
• Molecular Weight: 38.3kDa (340aa) 40-57KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: biglycan preproprotein
• NCBI Official Synonym Full Names: biglycan
• NCBI Official Symbol: BGN
• NCBI Official Synonym Symbols: PGI; MRLS; DSPG1; PG-S1; SEMDX; SLRR1A
• NCBI Protein Information: biglycan
• UniProt Protein Name: Biglycan
• Protein Family: Biglycan
• UniProt Gene Name: BGN
• UniProt Synonym Gene Names: SLRR1A

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, the encoded protein may contribute to atherosclerosis and aortic valve stenosis in human patients. This gene and the related gene decorin are thought to be the result of a gene duplication. [provided by RefSeq, Nov 2015]

Uniprot Description

May be involved in collagen fiber assembly.