Bacilysin biosynthesis oxidoreductase ywfH (ywfH) Recombinant Protein | ywfH recombinant protein
Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH (ywfH)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Bacilysin biosynthesis oxidoreductase ywfH (ywfH); Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH (ywfH); ywfH recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-259, Full length protein
Sequence
MSKRTAFVMGASQGIGKAIALKLADQHFSLVINSRNLDNIESVKEDILAKHPEASVIVLAGDMSDQHTRAGIFQKIESQCGRLDVLINNIPGGAPDTFDNCNIEDMTATFTQKTVAYIDAIKRASSLMKQNEFGRIINIVGNLWKEPGANMFTNSMMNAALINASKNISIQLAPHNITVNCLNPGFIATDRYHQFVENVMKKNSISKQKAEEQIASGIPMKRVGSAEETAALAAFLASEEASYITGQQISADGGSMKSI
Sequence Length
259
Species
Bacillus subtilis (strain 168)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,022 Da
NCBI Official Full Name
bacilysin biosynthesis oxidoreductase YwfH
NCBI Official Symbol
ywfH
NCBI Protein Information
bacilysin biosynthesis oxidoreductase YwfH
UniProt Protein Name
NADPH-dependent reductase BacG
UniProt Gene Name
bacG
Uniprot Description
Along with the bacABCDEF operon, BacG is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase (PubMed:20052993). BacG catalyzes the stereoselective reduction of exocyclic-delta3,delta5-dihydro-hydroxyphenylpyruvate (ex-H2HPP), adding a pro-S hydride equivalent to C4 position to yield tetrahydro-hydroxyphenylpyruvate (H4HPP) (PubMed:22765234, PubMed:23519407). Although the 3Z,7R-ex-H2HPP isomer is kinetically disfavored by BacB and produced in a smaller quantity than 3E,7R-ex-H2HPP, it is the preferred substrate for the conjugate reduction reaction of BacG (PubMed:22765234, PubMed:23519407).
Research Articles on ywfH
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Product Notes
The ywfH bacg (Catalog #AAA1168487) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-259, Full length protein. The amino acid sequence is listed below: MSKRTAFVMG ASQGIGKAIA LKLADQHFSL VINSRNLDNI ESVKEDILAK HPEASVIVLA GDMSDQHTRA GIFQKIESQC GRLDVLINNI PGGAPDTFDN CNIEDMTATF TQKTVAYIDA IKRASSLMKQ NEFGRIINIV GNLWKEPGAN MFTNSMMNAA LINASKNISI QLAPHNITVN CLNPGFIATD RYHQFVENVM KKNSISKQKA EEQIASGIPM KRVGSAEETA ALAAFLASEE ASYITGQQIS ADGGSMKSI. It is sometimes possible for the material contained within the vial of "Bacilysin biosynthesis oxidoreductase ywfH (ywfH), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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