Beta-1,4-galactosyltransferase 1  Recombinant Protein | B4GALT1 recombinant protein

Recombinant Bovine Beta-1,4-galactosyltransferase 1 

Cat. Num. AAA963398

• Gene Names: B4GALT1; GGTB2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Beta-1; 4-galactosyltransferase 1 ; Recombinant Bovine Beta-1; UDP-Gal: beta-GlcNAc beta-1; 4-galactosyltransferase 1; B4GALT1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-24aa; Cytoplasmic Domain
• Sequence: MKFREPLLGGSAAMPGASLQRACR
• Sequence Length: 24

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for B4GALT1 recombinant protein

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

References

Cloning of cDNA encoding the membrane-bound form of bovine beta 1,4-galactosyltransferase.D'Agostaro G., Bendiak B., Tropak M.Eur. J. Biochem. 183:211-217(1989) NIH - Mammalian Gene Collection (MGC) project Bovine galactosyltransferase identification of a clone by direct immunological screening of a cDNA expression library.Shaper N.L., Shaper J.H., Meuth J.L., Fox J.L., Chang H., Kirsch I.R., Hollis G.F.Proc. Natl. Acad. Sci. U.S.A. 83:1573-1577(1986) Cloning and sequencing of cDNA of bovine N-acetylglucosamine (beta 1-4) galactosyltransferase.Narimatsu H., Sinha S., Brew K., Okayama H., Qasba P.K.Proc. Natl. Acad. Sci. U.S.A. 83:4720-4724(1986) Qasba P.K., Narimatsu H., Masibay A.S.Expression of bovine beta-1,4-galactosyltransferase cDNA in COS-7 cells.Masibay A.S., Qasba P.K.Proc. Natl. Acad. Sci. U.S.A. 86:5733-5737(1989) Bovine beta 1-->4-galactosyltransferase two sets of mRNA transcripts encode two forms of the protein with different amino-terminal domains. In vitro translation experiments demonstrate that both the short and the long forms of the enzyme are type II membrane-bound glycoproteins.Russo R.N., Shaper N.L., Shaper J.H.J. Biol. Chem. 265:3324-3331(1990) Identification of a region of UDP-galactose:N-acetylglucosamine beta 4-galactosyltransferase involved in UDP-galactose binding by differential labeling.Yadav S.P., Brew K.J. Biol. Chem. 265:14163-14169(1990) Structure and function in galactosyltransferase. Sequence locations of alpha-lactalbumin binding site, thiol groups, and disulfide bond.Yadav S.P., Brew K.J. Biol. Chem. 266:698-703(1991) Crystal structures of the bovine beta4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose.Gastinel L.N., Cambillau C., Bourne Y.EMBO J. 18:3546-3557(1999) Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the beta1,4-galactosyltransferase-I.Ramakrishnan B., Qasba P.K.J. Mol. Biol. 310:205-218(2001) Crystal structure of beta1,4-galactosyltransferase complex with UDP-Gal reveals an oligosaccharide acceptor binding site.Ramakrishnan B., Balaji P.V., Qasba P.K.J. Mol. Biol. 318:491-502(2002) The role of tryptophan 314 in the conformational changes of beta1,4-galactosyltransferase-I.Ramasamy V., Ramakrishnan B., Boeggeman E., Qasba P.K.J. Mol. Biol. 331:1065-1076(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 29135321
• NCBI GeneID: 281781
• NCBI Accession #: NP_803478.1
• NCBI GenBank Nucleotide #: NM_177512.2
• UniProt Accession #: P08037; Q0VC05; Q8MIG0
• Molecular Weight: 29.5 kDa
• NCBI Official Full Name: beta-1,4-galactosyltransferase 1
• NCBI Official Symbol: B4GALT1
• NCBI Official Synonym Symbols: GGTB2
• NCBI Protein Information: beta-1,4-galactosyltransferase 1
• UniProt Protein Name: Beta-1,4-galactosyltransferase 1
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT1
• UniProt Synonym Gene Names: GALT; GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1
• UniProt Entry Name: B4GT1_BOVIN

Uniprot Description

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.