Beta-1,3-Galactosyltransferase 5 Recombinant Protein | b3GALT5 recombinant protein

Recombinant Beta-1,3-Galactosyltransferase 5 (b3GALT5)

Cat. Num. AAA2030762

• Gene Names: B3GALT5; B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Beta-1; 3-Galactosyltransferase 5; Recombinant Beta-1; 3-Galactosyltransferase 5 (b3GALT5); b3GALT5 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; NP FKEQSFVYKK DGNFLKLPDT DCRQTPPFLV LLVTSSHKQL AERMAIRQTW GKERMVKGKQ LKTFFLLGTT SSAAETKEVD QESQRHGDII QKDFLDVYYN LTLKTMMGIE WVHRFCPQAA FVMKTDSDMF INVDYLTELL LKKNRTTRFF TGFLKLNEFP IRQPFSKWFV SKSEYPWDRY PPFCSGTGYV FSGDVASQVY NVSKSVPYIK LEDVFVGLCL ERLNIRLEELHSQPTFFPGG LRFSVCLFRR IVACHFIKPR TLLDYWQALE NSRGEDCPPV
• Sequence Length: 310

• Applicable Applications for b3GALT5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 62.9kDa
• Accurate Molecular Mass (KD): 63kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Asn29~Val310
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 15451883
• NCBI GeneID: 10317
• NCBI Accession #: NP_149361.1
• NCBI GenBank Nucleotide #: NM_033171.2
• Molecular Weight: 36,189 Da
• NCBI Official Full Name: beta-1,3-galactosyltransferase 5 isoform a
• NCBI Official Synonym Full Names: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
• NCBI Official Symbol: B3GALT5
• NCBI Official Synonym Symbols: B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5
• NCBI Protein Information: beta-1,3-galactosyltransferase 5
• UniProt Protein Name: Beta-1,3-galactosyltransferase 5
• Protein Family: Beta-1,3-galactosyltransferase
• UniProt Gene Name: B3GALT5
• UniProt Synonym Gene Names: Beta-1,3-GalTase 5; Beta3Gal-T5; Beta3GalT5; b3Gal-T5
• UniProt Entry Name: B3GT5_HUMAN

NCBI Description

This gene encodes a member of a family of membrane-bound glycoproteins. The encoded protein may synthesize type 1 Lewis antigens, which are elevated in gastrointestinal and pancreatic cancers. Alternatively spliced transcript variants have been observed for this gene, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2013]

Uniprot Description

B3GALT5: Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc(beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor. Belongs to the glycosyltransferase 31 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; EC 2.4.1.-; Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Transferase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum; integral to membrane

Molecular Function: UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity

Biological Process: protein amino acid glycosylation