Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Anti beta 1 Adrenergic Receptor antibodies ELISA Kit | b1AR elisa kit
Guinea pig Anti beta 1 Adrenergic Receptor antibodies ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ANTI-B1AR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANTI-B1AR antibody and an ANTI-B1AR-HRP conjugate. The assay sample and buffer are incubated together with ANTI-B1AR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANTI-B1AR concentration since ANTI-B1AR from samples and ANTI-B1AR-HRP conjugate compete for the anti-ANTI-B1AR antibody binding site. Since the number of sites is limited, as more sites are occupied by ANTI-B1AR from the sample, fewer sites are left to bind ANTI-B1AR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANTI-B1AR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure. [provided by RefSeq, Jul 2008]
Uniprot Description
ADRB1: a GPCR-coupled 7 transmembrane receptor that binds epinephrine and norepinephrine with approximately equal affinity. Mediates the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Interacts with PIST and PSD-95. Localized at the plasma membrane and in the Golgi upon PIST overexpression. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 10q25.3
Cellular Component: integral to plasma membrane; early endosome; plasma membrane; nucleus
Molecular Function: protein binding; protein heterodimerization activity; receptor signaling protein activity; dopamine binding; drug binding; norepinephrine binding; epinephrine binding; beta-adrenergic receptor activity; alpha-2A adrenergic receptor binding; Ras guanyl-nucleotide exchange factor activity; PDZ domain binding; beta1-adrenergic receptor activity
Biological Process: fear response; diet induced thermogenesis; wound healing; positive regulation of apoptosis; apoptosis; lipid homeostasis; positive regulation of systemic arterial blood pressure; sensory perception of pain; positive regulation of saliva secretion; Rho protein signal transduction; regulation of inhibitory postsynaptic membrane potential; negative regulation of smooth muscle contraction; positive regulation of cAMP biosynthetic process; aging; glycogen catabolic process; negative regulation of multicellular organism growth; adenylate cyclase activation; regulation of calcium ion transport; memory; positive regulation of the force of heart contraction by norepinephrine; protein localization in organelle; heat generation; brown fat cell differentiation; response to cold; positive regulation of heart rate by epinephrine-norepinephrine; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure
Disease: Resting Heart Rate, Variation In
Research Articles on b1AR
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Product Notes
The Guinea Pig b1AR adrb1 (Catalog #AAA745435) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745435 ELISA Kit recognizes Guinea Pig b1AR. It is sometimes possible for the material contained within the vial of "Anti beta 1 Adrenergic Receptor antibodies, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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