Human Azurocidin ELISA Kit | AZU1 elisa kit

Human Azurocidin ELISA Kit

Cat. Num. AAA765905

• Reactivity: Human
• Synonyms: Azurocidin; Human Azurocidin ELISA Kit; AZU1 (Azurocidin 1)/AZAMP/AZU1/CAP37/HBP/AZAMP/AZU/azurocidin/azurocidin 1/Azurocidin/Cationic Antimicrobial Protein-37/Heparin Binding Protein; AZU1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AXIN1. No significant cross-reactivity or interference between AXIN1 and analogues was observed.
• Sequence Length: 251

• Samples: Cell, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AXIN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AXIN1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AZU1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AXIN1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AXIN1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AXIN1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AXIN1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 179302
• Molecular Weight: 26,886 Da
• NCBI Official Full Name: azurocidin
• UniProt Protein Name: Azurocidin
• Protein Family: Azurocidin
• UniProt Gene Name: AZU1
• UniProt Synonym Gene Names: hHBP
• UniProt Entry Name: CAP7_HUMAN

Uniprot Description

AZU1: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram- negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. Belongs to the peptidase S1 family. Elastase subfamily.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: azurophil granule; extracellular region; extracellular space; extrinsic to membrane

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; peptidase activity; protein binding; serine-type endopeptidase activity; toxin binding

Biological Process: antimicrobial humoral response; cellular extravasation; defense response to Gram-negative bacterium; defense response to virus; glial cell migration; induction of positive chemotaxis; inflammatory response; macrophage chemotaxis; microglial cell activation; monocyte activation; negative regulation of apoptosis; positive regulation of cell adhesion; positive regulation of fractalkine biosynthetic process; positive regulation of interleukin-1 beta biosynthetic process; positive regulation of MHC class II biosynthetic process; positive regulation of phagocytosis; positive regulation of protein kinase activity; positive regulation of tumor necrosis factor biosynthetic process; protein kinase C activation; protein processing; proteolysis; regulation of vascular permeability