Azurocidin/CAP37 ELISA Pair Set | AZU1 elisa pair set

Azurocidin/CAP37 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118283

• Synonyms: Azurocidin/CAP37; Azurocidin/CAP37 Matched ELISA Antibody Pair Set; Human; Human Azurocidin/CAP37 ELISA Pair Set; azurocidin 1; AZAMP Matched ELISA Antibody Pair Set; AZU Matched ELISA Antibody Pair Set; AZU1 Matched ELISA Antibody Pair Set; CAP37 Matched ELISA Antibody Pair Set; HBP Matched ELISA Antibody Pair Set; hHBP Matched ELISA Antibody Pair Set; HUMAZUR Matched ELISA Antibody Pair Set; NAZC Matched ELISA Antibody Pair Set; AZU1 elisa pair set

• Specificity: Quantitative determination of Human Azurocidin/CAP37

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 6.25-400pg/mL
• Sensitivity: 6.25pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human Azurocidin/CAP37 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Azurocidin/CAP37 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 12.5 ng of recombinant Human Azurocidin/CAP37. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for AZU1 elisa pair set

Azurocidin (AZU1), also known as heparin-binding protein (HBP) or cationic antimicrobial protein 37 (CAP37), is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. The Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. Azurocidin is a member of the serine protease family that includes Cathepsin G, neutrophil elastase (NE), and proteinase 3 (PR3), however, Azurocidin is not a serine proteinase since the active site serine and histidine residues are replaced. Neutrophils arriving first at sites of inflammation release Azurocidin, which acts in a paracrine fashion on endothelial cells causing the development of intercellular gaps and allowing leukocyte extravasation. It thus be regarded as a reasonable therapeutic target for a variety of inflammatory disease conditions.

This Azurocidin/CAP37 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Azurocidin/CAP37. It contains Human Azurocidin/CAP37 capture antibody, Human Azurocidin/CAP37 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Azurocidin/CAP37 protein.

References

Lindmark A, et al. (1999) Characterization of the biosynthesis, processing, and sorting of human HBP/CAP37/azurocidin. J Leukoc Biol. 66(4): 634-43.Heinzelmann M, et al. (2001) Heparin binding protein (CAP37) differentially modulates endotoxin-induced cytokine production. Int J Surg Investig. 2(6): 457-66.Soehnlein O, et al. (2005) Neutrophil-derived heparin-binding protein (HBP/CAP37) deposited on endothelium enhances monocyte arrest under flow conditions. J Immunol. 174(10): 6399-405.