Typical Testing Data/Standard Curve (for reference only)
Rat Axis Inhibition Protein ELISA Kit | AXIN elisa kit
Rat Axis Inhibition Protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AXIN ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-AXIN antibody and an AXIN-HRP conjugate. The assay sample and buffer are incubated together with AXIN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AXIN concentration since AXIN from samples and AXIN-HRP conjugate compete for the anti-AXIN antibody binding site. Since the number of sites is limited, as more sites are occupied by AXIN from the sample, fewer sites are left to bind AXIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AXIN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2010]
Uniprot Description
axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.
Protein type: Adaptor/scaffold; Tumor suppressor
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: Golgi apparatus; cytoplasmic microtubule; perinuclear region of cytoplasm; cytoplasm; cytoplasmic membrane-bound vesicle; postsynaptic density; cell cortex; cytoplasmic vesicle; nucleus; cytosol; beta-catenin destruction complex; lateral plasma membrane
Molecular Function: protein C-terminus binding; identical protein binding; protein homodimerization activity; protein self-association; p53 binding; beta-catenin binding; protein kinase binding; GTPase activator activity; protein binding; signal transducer activity; enzyme binding; receptor signaling complex scaffold activity; ubiquitin protein ligase binding; protein complex scaffold; SMAD binding; receptor binding
Biological Process: cell death; negative regulation of Wnt receptor signaling pathway; protein polyubiquitination; apoptosis; embryonic eye morphogenesis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; negative regulation of protein metabolic process; nucleocytoplasmic transport; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; olfactory placode formation; cytoplasmic microtubule organization and biogenesis; activation of JNK activity; sensory perception of sound; positive regulation of ubiquitin-protein ligase activity; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; protein catabolic process; protein homooligomerization; axial mesoderm formation; in utero embryonic development; muscle cell development; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of fat cell differentiation; optic placode formation; positive regulation of peptidyl-serine phosphorylation; cellular protein complex assembly; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; forebrain anterior/posterior pattern formation; activation of protein kinase activity; positive regulation of protein catabolic process; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; positive regulation of GTPase activity
Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma
Research Articles on AXIN
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Product Notes
The Rat AXIN axin1 (Catalog #AAA731951) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA731951 ELISA Kit recognizes Rat AXIN. It is sometimes possible for the material contained within the vial of "Axis Inhibition Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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