ATPase, Cu++ Transporting Alpha Polypeptide Recombinant Protein | ATP7a recombinant protein

Recombinant ATPase, Cu++ Transporting Alpha Polypeptide (ATP7a)

Cat. Num. AAA2123778

• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: ATPase; Cu++ Transporting Alpha Polypeptide; Recombinant ATPase; Cu++ Transporting Alpha Polypeptide (ATP7a); MK; MNK; MC1; Menkes Syndrome; Copper pump 1; Copper-transporting ATPase 1; Menkes disease-associated protein; ATP7a recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Ser9~Leu444
• Sequence Length: 1500

• Applicable Applications for ATP7a recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Subcellular Location: Membrane, Golgi apparatus
• Isoelectric Point: 5.8
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for ATP7a recombinant protein

Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 115529486
• NCBI Accession #: NP_000043.3
• NCBI GenBank Nucleotide #: NP_000043.3
• UniProt Accession #: Q04656
• Molecular Weight: Predicted Molecular Mass: 51kDa; Accurate Molecular Mass: 51kDa
• NCBI Official Full Name: copper-transporting ATPase 1
• UniProt Protein Name: Copper-transporting ATPase 1
• UniProt Gene Name: ATP7A
• UniProt Synonym Gene Names: MC1; MNK
• UniProt Entry Name: ATP7A_HUMAN

Uniprot Description

ATP7A: a multi-pass membrane protein and cation transporting ATPase. A copper-transporter that may supply copper to copper-requiring proteins within the secretory pathway when localized in the trans-Golgi network. Cycles between the trans-Golgi network (TGN) and the plasma membrane. Predominantly found in the TGN and relocalizes to the plasma membrane in response to elevated extracellular copper levels where it functions in the efflux of copper from cells. Defects in ATP7A are the cause of Menkes syndrome (MD) and occipital horn syndrome (OHS). Six alternatively spliced isoforms have been described. The longest isoform has 6 transmembrane regions. Isoform 3, lacking all 6 transmembrane regions and 5 heavy-metal-associated (HMA) domains, is probably cytosolic. Isoform 6 lacks all transmembrane regions and 5 heavy-metal-associated (HMA) domains, but has a putative nuclear localization signal attached at the N-terminus.

Protein type: Transporter, ion channel; Hydrolase; Vesicle; Cell development/differentiation; EC 3.6.3.54; Membrane protein, multi-pass; Membrane protein, integral; Chaperone; Apoptosis; Transporter

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: Golgi apparatus; neuron projection; brush border membrane; basolateral plasma membrane; endoplasmic reticulum; integral to membrane; trans-Golgi network; cytosol; secretory granule; trans-Golgi network transport vesicle; membrane; cell soma; perinuclear region of cytoplasm; late endosome; plasma membrane

Molecular Function: copper ion transmembrane transporter activity; protein binding; copper ion binding; copper-exporting ATPase activity; superoxide dismutase copper chaperone activity; ATP binding; copper-dependent protein binding

Biological Process: skin development; positive regulation of catalytic activity; extracellular matrix organization and biogenesis; collagen fibril organization; catecholamine metabolic process; dopamine metabolic process; norepinephrine biosynthetic process; tryptophan metabolic process; plasma membrane copper ion transport; negative regulation of neuron apoptosis; central nervous system neuron development; positive regulation of oxidoreductase activity; mitochondrion organization and biogenesis; release of cytochrome c from mitochondria; response to iron(III) ion; dendrite morphogenesis; detoxification of copper ion; ATP metabolic process; elastic fiber assembly; response to reactive oxygen species; response to zinc ion; regulation of gene expression; regulation of oxidative phosphorylation; cerebellar Purkinje cell differentiation; copper ion import; alveolus development; lactation; removal of superoxide radicals; locomotory behavior; pyramidal neuron development; norepinephrine metabolic process; peptidyl-lysine modification; epinephrine metabolic process; transmembrane transport; serotonin metabolic process; blood vessel development; negative regulation of metalloenzyme activity; hair follicle morphogenesis; cellular copper ion homeostasis; T-helper cell differentiation; in utero embryonic development; tyrosine metabolic process; positive regulation of metalloenzyme activity; copper ion transport; elastin biosynthetic process; pigmentation; cartilage development; blood vessel remodeling; copper ion export; neurite morphogenesis

Disease: Occipital Horn Syndrome; Menkes Disease; Spinal Muscular Atrophy, Distal, X-linked 3