ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) Recombinant Protein | ATP1b1 recombinant protein

Recombinant ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)

Cat. Num. AAA2011017

• Gene Names: Atp1b1; Atpb; Atp4b; Atpb-1; NKbeta1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: ATPase; Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1); Recombinant ATPase; ATP1b1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- TIQVMLLT ISELKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA YVLNIIRFLE KYKDSAQKDD MIFEDCGNVP SEPKERGDIN HERGERKVCR FKLDWLGNCS GLNDDSYGYR EGKPCIIIKL NRVLGFKPKP PKNESLETYP LMMKYNPNVL PVQCTGKRDE DKDKVGNIEY FGMGGYYGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTVD TEIRVECKAY GENIGYSEKD RFQGRFDVKI EIKS
• Sequence Length: 304

• Applicable Applications for ATP1b1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Thr53~Ser304 (Accession # Q545P0) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753138
• NCBI GeneID: 11931
• NCBI Accession #: NP_033851.1
• NCBI GenBank Nucleotide #: NM_009721.6
• UniProt Accession #: Q545P0; P14094
• Molecular Weight: 30.7kDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, beta 1 polypeptide
• NCBI Official Symbol: Atp1b1
• NCBI Official Synonym Symbols: Atpb; Atp4b; Atpb-1; NKbeta1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-1; sodium/potassium ATPase beta subunit; sodium/potassium-dependent ATPase subunit beta-1
• UniProt Protein Name: ATPase, Na+/K+ transporting, beta 1 polypeptide
• UniProt Gene Name: Atp1b1
• UniProt Entry Name: Q545P0_MOUSE

NCBI Description

This gene encodes an integral membrane protein that comprises a subunit of an ATP-metabolizing enzyme responsible for transporting sodium and potassium ions across the plasma membrane. This enzyme regulates the electrochemical gradient of these ions in cells, and plays a central role in osmoregulation and signal transmission in nerves and muscles, among other biological processes. The encoded protein is the non-catalytic beta subunit; it works together with a catalytic alpha subunit and a gamma subunit. [provided by RefSeq, Mar 2013]

Uniprot Description

ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter

Cellular Component: membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; intracellular; caveola; sodium:potassium-exchanging ATPase complex; sarcolemma; vesicle; myelin sheath

Molecular Function: protein binding; ATPase activator activity; sodium:potassium-exchanging ATPase activity; ATPase binding

Biological Process: blastocyst development; cellular sodium ion homeostasis; protein stabilization; sodium ion transport; cellular potassium ion homeostasis; cellular calcium ion homeostasis; metal ion transport; potassium ion import; regulation of gene expression; positive regulation of ATPase activity; transport; response to hypoxia; ion transport; cell adhesion; potassium ion transport; cardiac muscle contraction