Autophagy Related 3 Recombinant Protein | ATG3 recombinant protein

Recombinant Human Autophagy Related 3

Cat. Num. AAA556282

• Gene Names: ATG3; APG3; APG3L; PC3-96; APG3-LIKE
• Purity: Greater than 85.0% as determined by: Analysis by SDS-PAGE.
• Synonyms: Autophagy Related 3; Recombinant Human Autophagy Related 3; ATG3 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by: Analysis by SDS-PAGE.
• Form/Format: Liquid; ATG3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl and 10% glycerol.
• Sequence: MGSSHHHHHH SSGLVPRGSHMGSMQNVINT VKGKALEVAE YLTPVLKESK FKETGVITPE EFVAAGDHLV HHCPTWQWAT GEELKVKAYL PTGKQFLVTK NVPCYKRCKQ MEYSDELEAI IEEDDGDGGW VDTYHNTGIT GITEAVKEIT LENKDNIRLQ DCSALCEEEE DEDEGEAADM EEYEESGLLE TDEATLDTRK IVEACKAKTD AGGEDAILQT RTYDLYITYD KYYQTPRLWL FGYDEQRQPL TVEHMYEDIS QDHVKKTVTI ENHPHLPPPP MCSVHPCRHA EVMKKIIETV AEGGGELGVH MYLLIFLKFV QAVIPTIEYD YTRHFTM

• Species: Human
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.15% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for ATG3 recombinant protein

Autophagy Related 3 (ATG3) is a conjugating enzyme essential for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. ATG3 is localizes to the cytoplasm and is expressed in tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes a reaction which is necessary for autophagy. That reaction is the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate.

ATG3 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-314 a.a) and having a molecular mass of 38.3kDa.

ATG3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

NCBI and Uniprot Product Information

• NCBI GI #: 19526773
• NCBI GeneID: 64422
• NCBI Accession #: NP_071933
• NCBI GenBank Nucleotide #: NM_022488.4
• UniProt Accession #: Q9NT62; Q6PKC5; Q9H6L9
• Molecular Weight: 35,467 Da
• NCBI Official Full Name: ubiquitin-like-conjugating enzyme ATG3 isoform 1
• NCBI Official Synonym Full Names: autophagy related 3
• NCBI Official Symbol: ATG3
• NCBI Official Synonym Symbols: APG3; APG3L; PC3-96; APG3-LIKE
• NCBI Protein Information: ubiquitin-like-conjugating enzyme ATG3; hApg3; 2610016C12Rik; autophagy-related protein 3; ATG3 autophagy related 3 homolog
• UniProt Protein Name: Ubiquitin-like-conjugating enzyme ATG3
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG3
• UniProt Synonym Gene Names: APG3; APG3L; APG3-like; hApg3
• UniProt Entry Name: ATG3_HUMAN

NCBI Description

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG3: an E2-like enzyme essential for Apg8p lipidation.

Protein type: Enzyme, cellular metabolism; EC 6.3.2.-; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol

Molecular Function: protein binding; enzyme binding; small conjugating protein ligase activity; APG8 conjugating enzyme activity; APG12 conjugating enzyme activity

Biological Process: mitochondrion degradation; protein ubiquitination; protein modification process; mitochondrial fragmentation during apoptosis; protein targeting to membrane; autophagic vacuole formation