Guinea Pig Activating Transcription Factor 7 ELISA Kit | ATF7 elisa kit

Guinea Pig Activating Transcription Factor 7 ELISA Kit

Cat. Num. AAA720522

• Gene Names: ATF7; ATFA; MGC57182
• Reactivity: Guinea Pig
• Synonyms: Activating Transcription Factor 7; Guinea Pig Activating Transcription Factor 7 ELISA Kit; ATF7 elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This AFT7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig AFT7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATF7 elisa kit

Principle of the assay: AFT7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AFT7 antibody and an AFT7-HRP conjugate. The assay sample and buffer are incubated together with AFT7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AFT7 concentration since AFT7 from samples and AFT7-HRP conjugate compete for the anti-AFT7 antibody binding site. Since the number of sites is limited, as more sites are occupied by AFT7 from the sample, fewer sites are left to bind AFT7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFT7 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27503897
• NCBI GeneID: 11016
• UniProt Accession #: Q8IVR8; P17544
• Molecular Weight: 13,282 Da
• NCBI Official Full Name: ATF7 protein
• NCBI Official Synonym Full Names: activating transcription factor 7
• NCBI Official Symbol: ATF7
• NCBI Official Synonym Symbols: ATFA; MGC57182
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-7; transcription factor ATF-A
• UniProt Protein Name: ATF7 protein
• Protein Family: Activating transcription factor 7-interacting protein
• UniProt Gene Name: ATF7
• UniProt Entry Name: Q8IVR8_HUMAN

Uniprot Description

ATF7: Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]- 3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Belongs to the bZIP family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; sequence-specific DNA binding; metal ion binding; mitogen-activated protein kinase binding; transcription factor activity; transcription factor binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; viral reproduction; negative regulation of transcription from RNA polymerase II promoter