ATF2 / Cyclic AMP-Dependent Transcription Factor ATF-2 Recombinant Protein | ATF2 recombinant protein

Human ATF2 / Cyclic AMP-Dependent Transcription Factor ATF-2 Recombinant Protein

• Gene Names: ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: ATF2 / Cyclic AMP-Dependent Transcription Factor ATF-2; Human ATF2 / Cyclic AMP-Dependent Transcription Factor ATF-2 Recombinant Protein; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1; CREB2; CREBP1; ATF2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 505

• Applicable Applications for ATF2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Heterodimerization is essential for its transcriptional activity. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Interacts with the HK1/VDAC1 complex. Interacts with NBN, MRE11, XPO1, KAT5 and CUL3.
• Entry Function: Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 368711269
• NCBI GeneID: 1386
• NCBI Accession #: NP_001243019.1
• NCBI GenBank Nucleotide #: NP_001243019.1
• UniProt Accession #: P15336
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-2 isoform 1
• NCBI Official Synonym Full Names: activating transcription factor 2
• NCBI Official Symbol: ATF2
• NCBI Official Synonym Symbols: HB16; CREB2; TREB7; CREB-2; CRE-BP1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-2
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-2
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: ATF2
• UniProt Synonym Gene Names: CREB2; CREBP1; cAMP-dependent transcription factor ATF-2; CREB-2
• UniProt Entry Name: ATF2_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Uniprot Description

ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.

Protein type: EC 2.3.1.48; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: nucleoplasm; mitochondrial outer membrane; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; histone acetyltransferase activity; protein heterodimerization activity; metal ion binding; transcription coactivator activity; cAMP response element binding protein binding; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to osmotic stress; positive regulation of transforming growth factor-beta2 production; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription, DNA-dependent; regulation of transcription factor activity; intra-S DNA damage checkpoint; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; histone acetylation; toll-like receptor 4 signaling pathway; response to DNA damage stimulus