Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cyclic AMP-dependent transcription factor ATF-2 (ATF2) Standard Curve.)
Mouse Cyclic AMP-Dependent Transcription Factor ATF-2 (ATF2) ELISA Kit | ATF2 elisa kit
Mouse Cyclic AMP-Dependent Transcription Factor ATF-2 (ATF2) ELISA Kit
Gene Names
Atf2; mXBP; Atf-2; Creb2; CRE-BP; D18875; D130078H02Rik; Tg(Gzma-Klra1)7Wum
Reactivity
Mouse
Synonyms
Cyclic AMP-Dependent Transcription Factor ATF-2 (ATF2); Mouse Cyclic AMP-Dependent Transcription Factor ATF-2 (ATF2) ELISA Kit; ATF2; CRE-BP1; CREB2; HB16; MGC111558; TREB7; activating transcription factor 2 splice variant ATF2-var2; cAMP responsive element binding protein 2; formerly; ATF2 elisa kit
Reactivity
Mouse
Specificity
Mouse Cyclic AMP-dependent transcription factor ATF-2 (ATF2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ATF2. No significant cross-reactivity or interference between Mouse ATF2 and analogues was observed.
Sequence Length
487
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cyclic AMP-dependent transcription factor ATF-2 (ATF2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cyclic AMP-dependent transcription factor ATF-2 (ATF2) Standard Curve.)
Related Product Information for ATF2 elisa kit
Description: This Mouse Cyclic AMP-dependent transcription factor ATF-2 (ATF2) ELISA Kit employs a two-site sandwich ELISA to quantitate ATF2 in samples. An antibody specific for ATF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Glial Cell Line-derived Neurotrophic Factor (GDNF) is a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy.The protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
Background: Glial Cell Line-derived Neurotrophic Factor (GDNF) is a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy.The protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-2 isoform 1
NCBI Official Synonym Full Names
activating transcription factor 2
NCBI Official Symbol
Atf2
NCBI Official Synonym Symbols
mXBP; Atf-2; Creb2; CRE-BP; D18875; D130078H02Rik; Tg(Gzma-Klra1)7Wum
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-2
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-2
Protein Family
UniProt Gene Name
Atf2
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-2
UniProt Entry Name
ATF2_MOUSE
Research Articles on ATF2
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Product Notes
The Mouse ATF2 atf2 (Catalog #AAA9718111) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718111 ELISA Kit recognizes Mouse ATF2. It is sometimes possible for the material contained within the vial of "Cyclic AMP-Dependent Transcription Factor ATF-2 (ATF2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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