ATF2 recombinant protein

ATF2 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120868

• Gene Names: ATF2; HB16; CREB2; TREB7; CRE-BP1
• Purity: >80% as determined by SDS-PAGE
• Synonyms: ATF2; ATF2 Protein; Human; Recombinant (His & GST Tag); Human ATF2 Protein (His & GST Tag); CRE-BP1 Protein; CREB-2 Protein; CREB2 Protein; HB16 Protein; TREB7 Protein; activating transcription factor 2; ATF2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser505

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding full length of human ATF2 (P15336-1)(Met 1-Ser 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ATF2 recombinant protein

Background: Activating transcription factor 2, also known as ATF2, is a member of the leucine zipper family of DNA-binding proteins that binds to the cAMP response element. Its activity is enhanced after phosphorylation by stress-activated protein kinases such as c-Jun N-terminal kinase and p38. ATF2 has been found to be a target of the JNK signal transduction pathway and mediate adenovirus E1A-inducible transcriptional activation. ATF2 is also been reported playing roles in TGF-beta signaling pathway. It has been shown that the transcription factor ATF2 is bound by a hetero-oligomer of Smad3 and Smad4 upon TGF-beta stimulation. Studies indicate that ATF-2 plays a central role in TGF-beta signaling by acting as a common nuclear target of both Smad and TAK1 pathways.

Product Categories/Family for ATF2 recombinant protein

Non-Canonical Wnt Pathway

References

Livingstone C, et al. (1995) ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14 (8): 1785-97.Gupta S, et al. (1995) Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science. 267 (5196): 389-93.Sano YJ, et al. (1999) ATF-2 Is a Common Nuclear Target of Smad and TAK1 Pathways in Transforming Growth Factor-_ Signaling. The Journal of Biological Chemistry. 274: 8949-57.

NCBI and Uniprot Product Information

• NCBI GI #: 368711269
• NCBI GeneID: 1386
• NCBI Accession #: NP_001243019.1
• NCBI GenBank Nucleotide #: NM_001256090.1
• UniProt Accession #: P15336; Q13000; Q4ZFU9; Q53RY2; Q8TAR1; A1L3Z2; D3DPE9
• Molecular Weight: 54,537 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-2 isoform 1
• NCBI Official Synonym Full Names: activating transcription factor 2
• NCBI Official Symbol: ATF2
• NCBI Official Synonym Symbols: HB16; CREB2; TREB7; CRE-BP1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-2; CREB-2; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; cAMP response element-binding protein CRE-BP1; cyclic AMP-responsive element-binding protein 2; cAMP responsive
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-2
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: ATF2
• UniProt Synonym Gene Names: CREB2; CREBP1; cAMP-dependent transcription factor ATF-2; CREB-2
• UniProt Entry Name: ATF2_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.

Protein type: EC 2.3.1.48; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: nucleoplasm; mitochondrial outer membrane; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; histone acetyltransferase activity; protein heterodimerization activity; metal ion binding; transcription coactivator activity; cAMP response element binding protein binding; chromatin binding; transcription factor activity; protein kinase binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to osmotic stress; positive regulation of transforming growth factor-beta2 production; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription, DNA-dependent; regulation of transcription factor activity; intra-S DNA damage checkpoint; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; histone acetylation; toll-like receptor 4 signaling pathway; response to DNA damage stimulus