Human Activating Transcription Factor 6 ELISA Kit | ATF-6 elisa kit

Human Activating Transcription Factor 6 ELISA Kit

Cat. Num. AAA761043

• Gene Names: ATF6; ACHM7; ATF6A
• Reactivity: Human
• Synonyms: Activating Transcription Factor 6; Human Activating Transcription Factor 6 ELISA Kit; ATF-6; ATF-6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATF-6. No significant cross-reactivity or interference between ATF-6 and analogues was observed.
• Sequence Length: 670

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATF-6 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrate s were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Re ad the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119611100
• NCBI GeneID: 22926
• Molecular Weight: 74,585 Da
• NCBI Official Full Name: activating transcription factor 6
• NCBI Official Synonym Full Names: activating transcription factor 6
• NCBI Official Symbol: ATF6
• NCBI Official Synonym Symbols: ACHM7; ATF6A
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-6 alpha
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-6 alpha
• UniProt Gene Name: ATF6
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
• UniProt Entry Name: ATF6A_HUMAN

NCBI Description

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Uniprot Description

ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.

Protein type: Membrane protein, integral; Transcription factor

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; integral to endoplasmic reticulum membrane; membrane; nuclear envelope; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity; ubiquitin protein ligase binding

Biological Process: cellular protein metabolic process; eye development; positive regulation of transcription of target genes involved in unfolded protein response; protein folding; regulation of transcription from RNA polymerase II promoter; response to stress; signal transduction; transcription, DNA-dependent; unfolded protein response; visual perception

Disease: Achromatopsia 7