Human Activating Transcription Factor 2 ELISA Kit | ATF-2 elisa kit

Human Activating Transcription Factor 2 ELISA Kit

Cat. Num. AAA760697

• Gene Names: ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
• Reactivity: Human
• Synonyms: Activating Transcription Factor 2; Human Activating Transcription Factor 2 ELISA Kit; ATF-2; ATF-2 elisa kit

• Reactivity: Human
• Sequence Length: 505

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ATF-2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human ATF-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ATF-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ATF-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ATF-2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 120660330
• NCBI GeneID: 1386
• Molecular Weight: 15,409 Da
• NCBI Official Full Name: Activating transcription factor 2
• NCBI Official Synonym Full Names: activating transcription factor 2
• NCBI Official Symbol: ATF2
• NCBI Official Synonym Symbols: HB16; CREB2; TREB7; CREB-2; CRE-BP1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-2
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-2
• UniProt Gene Name: ATF2
• UniProt Synonym Gene Names: CREB2; CREBP1; cAMP-dependent transcription factor ATF-2; CREB-2; cAMP-responsive element-binding protein 2
• UniProt Entry Name: ATF2_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Uniprot Description

ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.

Protein type: Transcription factor; EC 2.3.1.48; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: cytoplasm; mitochondrial outer membrane; nucleoplasm; nucleus

Molecular Function: cAMP response element binding protein binding; chromatin binding; histone acetyltransferase activity; metal ion binding; protein binding; protein heterodimerization activity; protein kinase binding; RNA polymerase II transcription factor activity, enhancer binding; transcription coactivator activity; transcription factor activity

Biological Process: establishment and/or maintenance of chromatin architecture; fat cell differentiation; histone acetylation; innate immune response; intra-S DNA damage checkpoint; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of neuron apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transforming growth factor-beta2 production; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to osmotic stress; response to water deprivation; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter