NADH Oxidase (NOX) Assay Kit | NOX assay kit
NADH Oxidase (NOX) Colorimetric Assay Kit
Synonyms
NADH Oxidase (NOX); NADH Oxidase (NOX) Colorimetric Assay Kit; NOX assay kit
Kit Components
Sample Lysis Buffer
Mitochondria Lysis Buffer
Protease Inhibitor
Chromogen
Substrate
Mitochondria Lysis Buffer
Protease Inhibitor
Chromogen
Substrate
Features & Benefits
Determination of NADH oxidase (NOX) activity in tissue/cell lysates and other biological fluids.
Kit components and procedures are simple.
Sample preparation, experimental steps and result calculation are more detailed and accurate.
Long shelf life.
Kit components and procedures are simple.
Sample preparation, experimental steps and result calculation are more detailed and accurate.
Long shelf life.
Usage Notes
Assay kit is intended for research use only. Not for use in test procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
Avoid foaming or bubbles when mixing or reconstituting components.
Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
Ensure all reagents and equipment are at the appropriate temperature before assay.
The sample preparation must be done at 0 degree C-4 degree C to prevent the enzyme inactivation.
During the experiment, Substrate and samples should be placed on ice to avoid enzyme inactivation.
The reaction temperature should be constant, the experiment needs to be conducted in a thermostat.
It is better for two people to do this experiment at the same time, one to measure results and one to record time to ensure the accuracy of the experimental results.
Since the enzyme activity is calculated based on the reaction rate, when using a 96-well plate, please control the number of samples to be measured at one time according to the operating speed.
It is recommended to calculate the enzyme activity by sample protein concentration. If the sample fresh weight is used, it needs to detect the cytoplasmic extract enzyme activity as well. The sum of the supernatant and precipitation enzyme activity is the total enzyme activity.
Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
Avoid foaming or bubbles when mixing or reconstituting components.
Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
Ensure all reagents and equipment are at the appropriate temperature before assay.
The sample preparation must be done at 0 degree C-4 degree C to prevent the enzyme inactivation.
During the experiment, Substrate and samples should be placed on ice to avoid enzyme inactivation.
The reaction temperature should be constant, the experiment needs to be conducted in a thermostat.
It is better for two people to do this experiment at the same time, one to measure results and one to record time to ensure the accuracy of the experimental results.
Since the enzyme activity is calculated based on the reaction rate, when using a 96-well plate, please control the number of samples to be measured at one time according to the operating speed.
It is recommended to calculate the enzyme activity by sample protein concentration. If the sample fresh weight is used, it needs to detect the cytoplasmic extract enzyme activity as well. The sum of the supernatant and precipitation enzyme activity is the total enzyme activity.
Preparation and Storage
Storage at -20 degree C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Related Product Information for NOX assay kit
NADH Oxidase (NOX) Colorimetric Assay Kit is designed for detecting NADH Oxidase activity in the sample.
Background: NOX (EC 1.6.99.3) is widely found in animals, plants, microorganisms and cultured cells, and can directly oxidize NADH to NAD in the presence of oxygen. This enzyme is not only involved in the regeneration of NAD, but also closely related to the immune response.
Background: NOX (EC 1.6.99.3) is widely found in animals, plants, microorganisms and cultured cells, and can directly oxidize NADH to NAD in the presence of oxygen. This enzyme is not only involved in the regeneration of NAD, but also closely related to the immune response.
Product Categories/Family for NOX assay kit
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Product Notes
The NOX (Catalog #AAA9719072) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NADH Oxidase (NOX), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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