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Immunofluorescence (IF)

Mycoplasma Staining Assay Kit

Mycoplasma Staining Kit

Synonyms
Mycoplasma Staining; Mycoplasma Staining Kit; Mycoplasma Staining assay kit
Ordering
For Research Use Only!
Specificity
Detection of Mycoplasma in both suspension and adherent cell cultures
Sample Type
Adherent and suspension cell cultures
Features & Benefits
Ready-to-use, Ultrasensitive, Rapid, results in 60 min or less, Visual identification
Preparation and Storage
Store at 4 degree C
Shipping: Gel Pack
Shelf Life: Est.~6 months

Immunofluorescence (IF)

Immunofluorescence (IF)
Related Product Information for Mycoplasma Staining assay kit
Mycoplasma Staining Kit provides anultrasensitive, rapid and simple fluorescence based microscopic assay for the visual identification of mycoplasma infections in laboratory cell cultures. Mycoplasma infections are relatively common in laboratory cell cultures. It has been estimated that between 5% and 35% of all cell cultures are infected. Mycoplasmas have been shown to alter the growth rate of cells in culture, induce chromosomal aberrations, influence amino acid and nucleic acid metabolism and cause membrane aberrations. Mycoplasma Staining Kit contains a MycoFluor Reagent and Antifade Mounting Medium. The MycoFluor reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope with an excitation and emission maxima of 350-360nM and 450-460nM respectively. Mycoplasma staining with MycoFluor reagent appears as fine particulate or filamentous staining in the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.
Detection of mycoplasma by a fluorescence based microscopic assay

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Product Notes

The Mycoplasma Staining (Catalog #AAA8400616) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mycoplasma Staining, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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