CytoSelect Cell Contraction Assay Kit

CytoSelect 24-Well Cell Contraction Assay Kit (Floating Matrix Model)

Cat. Num. AAA169267

• Synonyms: CytoSelect Cell Contraction; CytoSelect 24-Well Cell Contraction Assay Kit (Floating Matrix Model); CytoSelect Cell Contraction assay kit

• Application Notes: Includes optional cell contraction inhibitor
• Preparation and Storage: Store all components at 4 degree C.

Related Product Information for CytoSelect Cell Contraction assay kit

Background/Introduction: Wound healing is comprised of three processes: epithelialization, connective tissue deposition, and contraction. The contraction process is believed to be mediated by specialized fibroblasts called myofibroblasts. Three-dimensional collagen gels have been widely used to study fibroblast contraction, integrin signaling, cell apoptosis and cytoskeleton reorganization. Since three-dimensional matrix adhesions differ in structure, localization, and function from two-dimensional adhesions; and therefore, three-dimensional cell-matrix interactions may be more relevant biologically. The signaling mechanisms used by fibroblasts to regulate collagen matrix contraction depend on whether the cells are mechanically loaded or unloaded at the time that contraction is initiated as well as on the growth factor used to initiate contraction. For instance, stimulation of fibroblasts by lysophosphatidic acid (LPA) but not by platelet-derived growth factor (PDGF) causes robust force generation in restrained matrices, whereas LPA and PDGF stimulate floating matrix contraction equally well. There are several different culture models to study the ability of fibroblasts to reorganize and contract collagen matrices in vitro. In the attached model, a polymerized collagen matrix containing cells remains attached to a culture dish during contraction. Mechanical tension develops during contraction, and cellular stress fibers assemble. The two-step model combines an initial period of attached matrix contraction leading to mechanical loading, followed by release of the matrices, resulting in mechanical unloading and further contraction as mechanical stress dissipates. In the floating matrix contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. CytoSelect 24-well Cell Contraction Assay Kit (Floating Matrix Model) provides a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The kit's proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. Each kit contains sufficient quantities to perform up to 24 assays in the provided 24-well plate.

Product Categories/Family for CytoSelect Cell Contraction assay kit

Cell Based Assays; Cell Contraction Assays; Cell Contraction Assays; Floating Matrix Model