BCA Protein Assay Kit
BCA Protein Assay Kit
Synonyms
BCA Protein Assay Kit; BCA Protein Assay Kit assay kit
Related Product Information for BCA Protein Assay Kit assay kit
Principle of the Assay: In an alkaline medium, Cu2+ was reduced to Cu+ by protein, and the purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with Cu+. This complex exhibits a strong absorbance at 562nm, and since the absorbancy strength is proportional to the protein concentration, determine the absorption value at 562nm, then compare with the standard curve, the protein concentration can be calculated.!!Background/Introduction: BCA (bicinchoninic acid) is a perfect protein assay method, it serves the purpose of the Folin reagent in the Lowry assay, namely to react with complexes between copper ions and peptide bonds to produce a purple end product. This method is favored by professionals for its high sensitivity, stability, reliability, and small variation coefficient on different kinds of protein detections. Determination of the protein concentration with BCA is not influenced by chemical substances in majority of the samples. For example, in tissue and cell lysate experiments, the test results were not affected by common detergents (SDS, Triton X-100, Tween), but will be influenced by chelant (EDTA, EGTA), deoxidizer (DTT, mercaptoethanol) and lipid. So, in the assay, if the background of the sample diluent buffer or lysis buffer is high, end user can try to use Bradford Protein Assay Kit (Catalog # MBS355526). This kit can work well here.
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Product Notes
The BCA Protein Assay Kit (Catalog #AAA355531) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BCA Protein Assay Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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