ASL recombinant protein

ASL, 1-464aa, Human, His tag, E Coli

Cat. Num. AAA204546

• Gene Names: ASL; ASAL
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ASL; 1-464aa; Human; His tag; E Coli; Argininosuccinate lyase; ASAL; ASL recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MASESGKLWG GRFVGAVDPI MEKFNASIAY DRHLWEVDVQ GSKAYSRGLE KAGLLTKAEM DQILHGLDKV AEEWAQGTFK LNSNDEDIHT ANERRLKELI GATAGKLHTG RSRNDQVVTD LRLWMRQTCS TLSGLLWELI RTMVDRAEAE RDVLFPGYTH LQRAQPIRWS HWILSHAVAL TRDSERLLEV RKRINVLPLG SGAIAGNPLG VDRELLRAEL NFGAITLNSM DATSERDFVA EFLFWASLCM THLSRMAEDL ILYCTKEFSF VQLSDAYSTG SSLMPQKKNP DSLELIRSKA GRVFGRCAGL LMTLKGLPST YNKDLQEDKE AVFEVSDTMS AVLQVATGVI STLQIHQENM GQALSPDMLA TDLAYYLVRK GMPFRQAHEA SGKAVFMAET KGVALNQLSL QELQTISPLF SGDVICVWDY GHSVEQYGAL GGTARSSVDW QIRQVRALLQ AQQA
• Sequence Length: 464

• Applicable Applications for ASL recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ASL recombinant protein

ASL (argininosuccinate lyase) belongs to the lyase 1 family. ASL is an enzyme that catalyzes the reversible breakdown of argininosuccinate (ASA) producing the amino acids arginine and fumarate. Located in liver cytosol, ASL is the fourth enzyme of the urea cycle and involved in the biosynthesis of arginine in all species and the production of urea in ureotelic species. While ASS (argininosuccinate synthetase) catalyzes the formation of argininosuccinate from citrulline and aspartate, ASL breaks the newly formed argininosuccinate into L-arginine and fumarate. L-arginine continues through the urea cycle to form urea and orinthine, while fumarate can enter the citric acid cycle. Recombinant human ASL protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ASL recombinant protein

Metabolism

References

Barbosa P, et al. (2008). J Biol Chem. 266 (8):5286-90; Zhao S., et al (2010) Science 327:1000-1004

NCBI and Uniprot Product Information

• NCBI GI #: 31541964
• NCBI GeneID: 435
• NCBI Accession #: NP_000039
• NCBI GenBank Nucleotide #: NM_000048.3
• UniProt Accession #: P04424; Q6LDS5; Q96HS2; E7EMI0; E9PE48
• Molecular Weight: 53.8kDa (484aa)
• NCBI Official Full Name: argininosuccinate lyase isoform 1
• NCBI Official Synonym Full Names: argininosuccinate lyase
• NCBI Official Symbol: ASL
• NCBI Official Synonym Symbols: ASAL
• NCBI Protein Information: argininosuccinate lyase
• UniProt Protein Name: Argininosuccinate lyase
• UniProt Gene Name: ASL
• UniProt Synonym Gene Names: ASAL

NCBI Description

This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]