Typical Testing Data/Standard Curve (for reference only)
Chicken Arsenic (+3 oxidation state) methyltransterase ELISA Kit | AS3MT elisa kit
Chicken Arsenic (+3 oxidation state) methyltransterase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AOXMT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AOXMT antibody and an AOXMT-HRP conjugate. The assay sample and buffer are incubated together with AOXMT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOXMT concentration since AOXMT from samples and AOXMT-HRP conjugate compete for the anti-AOXMT antibody binding site. Since the number of sites is limited, as more sites are occupied by AOXMT from the sample, fewer sites are left to bind AOXMT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOXMT concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM, Mar 2008]
Uniprot Description
AS3MT: Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me- AsO(3)H(2), which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me(2)As(O)-OH. Belongs to the methyltransferase superfamily.
Protein type: Mitochondrial; Methyltransferase; EC 2.1.1.137
Chromosomal Location of Human Ortholog: 10q24.32
Cellular Component: mitochondrion; cytoplasm; cytosol
Molecular Function: arsenite methyltransferase activity; methylarsonite methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity
Biological Process: methylation; arsonoacetate metabolic process; toxin metabolic process
Research Articles on AS3MT
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Product Notes
The Chicken AS3MT as3mt (Catalog #AAA755362) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755362 ELISA Kit recognizes Chicken AS3MT. It is sometimes possible for the material contained within the vial of "Arsenic (+3 oxidation state) methyltransterase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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