SDS-PAGE
ART4 / CD297 recombinant protein
Recombinant Mouse ART4 / CD297 Protein (His tag)
Gene Names
Art4; DO; DOK1; ARTC4; 4432404K01Rik
Purity
> 92 % as determined by SDS-PAGE
Synonyms
ART4 / CD297; Recombinant Mouse ART4 / CD297 Protein (His tag); 4432404K01Rik; DO; DOK1; ART4 / CD297 recombinant protein
Host
Human Cells
Purity/Purification
> 92 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
300
Application Notes
The recombinant mouse ART4 consists of 251 amino acids and predicts a molecular mass of 28.2 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmART4 is approximately 40-45 KDa as a result of glycosylation.
Predicted N Terminal
Gly 24
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for ART4 / CD297 recombinant protein
Background: ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4 (ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E Coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.
Description: A DNA sequence encoding the mature form of mouse ART4 (EDL10575.1) (Met1-Lys 263) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the mature form of mouse ART4 (EDL10575.1) (Met1-Lys 263) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,080 Da
NCBI Official Full Name
ADP-ribosyltransferase 4
NCBI Official Synonym Full Names
ADP-ribosyltransferase 4
NCBI Official Symbol
Art4
NCBI Official Synonym Symbols
DO; DOK1; ARTC4; 4432404K01Rik
NCBI Protein Information
ecto-ADP-ribosyltransferase 4
UniProt Protein Name
Ecto-ADP-ribosyltransferase 4
UniProt Gene Name
Art4
UniProt Synonym Gene Names
ARTC4
Uniprot Description
ART4: a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known. [provided by RefSeq, Jul 2008]
Protein type: EC 2.4.2.31; Membrane protein, GPI anchor; Transferase
Chromosomal Location of Human Ortholog: 6 G1|6 66.72 cM
Similar Products
Product Notes
The ART4 / CD297 art4 (Catalog #AAA2546665) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse ART4 consists of 251 amino acids and predicts a molecular mass of 28.2 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmART4 is approximately 40-45 KDa as a result of glycosylation. Researchers should empirically determine the suitability of the ART4 / CD297 art4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ART4 / CD297, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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