Human Arrestin 1 ELISA Kit | ARRB1 elisa kit

Arrestin 1 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500041

• Gene Names: ARRB1; ARB1; ARR1
• Reactivity: Human
• Synonyms: Arrestin 1; Arrestin 1 Colorimetric Cell-Based ELISA Kit; ARR1; arrestin 2; arrestin beta 1; beta-arrestin-1; ARRB1 elisa kit

• Reactivity: Human
• Form/Format: One 96-Well Plate
• Sequence Length: 418

• Samples: Researchers plate their cell line of choice
• Assay Type: Non-Phospho Cell-Based ELISA
• Sensitivity: >=5000 cells/well
• Detection Method: Colorimetric 450 nm
• Preparation and Storage: Store at 4 degree C.

Product Categories/Family for ARRB1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 10880136
• NCBI GeneID: 408
• NCBI Accession #: NP_004032.2
• NCBI GenBank Nucleotide #: NM_004041.4
• UniProt Accession #: P49407; O75625; O75630; Q2PP20; Q9BTK8; B6V9G8
• Molecular Weight: 47066
• NCBI Official Full Name: beta-arrestin-1 isoform A
• NCBI Official Synonym Full Names: arrestin beta 1
• NCBI Official Symbol: ARRB1
• NCBI Official Synonym Symbols: ARB1; ARR1
• NCBI Protein Information: beta-arrestin-1
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB1
• UniProt Synonym Gene Names: ARR1

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: basolateral plasma membrane; chromatin; clathrin-coated pit; cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; cytosol; dendritic spine; Golgi membrane; heterotrimeric G-protein complex; lysosomal membrane; nuclear body; nucleoplasm; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane; pseudopodium

Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; caspase inhibitor activity; enzyme inhibitor activity; estrogen receptor binding; follicle-stimulating hormone receptor binding; GTPase activator activity; histone acetyltransferase activity; insulin-like growth factor receptor binding; ion channel binding; mitogen-activated protein kinase kinase binding; protein binding; protein phosphorylated amino acid binding; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding; V2 vasopressin receptor binding

Biological Process: activation of MAPK activity; apoptosis; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; G-protein coupled receptor signaling pathway; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of ERK1 and ERK2 cascade; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of NF-kappaB transcription factor activity; negative regulation of Notch signaling pathway; negative regulation of protein ubiquitination; phototransduction; platelet activation; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of ERK1 and ERK2 cascade; positive regulation of GTPase activity; positive regulation of histone acetylation; positive regulation of histone H4 acetylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein phosphorylation; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; positive regulation of smooth muscle cell apoptotic process; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; response to drug; stress fiber assembly; transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process