Rho GTPase-activating protein 35 (ARHGAP35) Recombinant Protein | ARHGAP35 recombinant protein

Recombinant Human Rho GTPase-activating protein 35 (ARHGAP35) , partial

• Gene Names: ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Rho GTPase-activating protein 35 (ARHGAP35); Recombinant Human Rho GTPase-activating protein 35 (ARHGAP35); partial; ARHGAP35 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,499

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for ARHGAP35 recombinant protein

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.

NCBI and Uniprot Product Information

• NCBI GI #: 150417981
• NCBI GeneID: 2909
• NCBI Accession #: NP_004482.4
• NCBI GenBank Nucleotide #: NM_004491.4
• UniProt Accession #: Q9NRY4; Q14452; Q9C0E1; A7E2A4
• Molecular Weight: 170,514 Da
• NCBI Official Full Name: rho GTPase-activating protein 35
• NCBI Official Synonym Full Names: Rho GTPase activating protein 35
• NCBI Official Symbol: ARHGAP35
• NCBI Official Synonym Symbols: GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
• NCBI Protein Information: rho GTPase-activating protein 35
• UniProt Protein Name: Rho GTPase-activating protein 35
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: ARHGAP35
• UniProt Synonym Gene Names: GRF-1; p190-A

NCBI Description

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]

Uniprot Description

Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621).